摘要
目的探讨黄芪多糖水溶液对人牙周膜细胞增殖、分化及结构的影响。方法体外培养人牙周膜细胞,检测在黄芪多糖质量浓度为0.08、0.1、0.2、0.4 mg.mL-1的培养基中细胞增殖与结构。结果当黄芪多糖质量浓度为0.2 mg·mL-1时,甲基噻唑基四唑(MTT)法所测吸光度值显著高于对照组(P<0.05),而在这一质量浓度下,牙周膜细胞碱性磷酸酶的表达明显高于对照组,同时细胞能保持良好的表面结构和超微结构。结论适宜质量浓度黄芪多糖水溶液在短期内对体外牙周膜细胞的增殖具有一定的促进作用。
Objective To investigate the effect of Astragalus membranaceus（APS） on the proliferation,osteogenic capacity and structure of periodontal ligament cells（PDLCs） in vitro.Methods PDLCs were cultured in vitro with APS of 0.08、 0.1、 0.2、 0.4 mg·mL-1.Methyl thiazolyl tetrazolium（MTT）,alkaline phosphatase（ALP） and cell structure were detected to determine the proliferation and differentiation of PDLCs proliferation and differentiation.Results When the APS was 0.2 mg.mL-1,the absorbance of MTT and ALP exhibit significantly increased as compared to the contro（lP0.05）.The cells cultured in vitro with APS of 0.2 mg·mL-1 had the normal structure.Conclusion APS with proper concentration in short-term culture may promote the proliferation and differentiation of PDLCs.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2010年第5期556-559,共4页
West China Journal of Stomatology
基金
四川省科技支撑计划基金资助项目(2009FZ0062)
关键词
黄芪多糖
牙周膜细胞
增殖
Astragalus membranaceus
periodontal ligament cells
proliferation
