摘要
目的研究在中国人汉族人群中,子宫肌瘤的发生与雌激素受体β(estrogen receptor,ERβ)基因多态性之间的关系。方法自2007年1月至2008年6月,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)来扩增ERβ基因序列,用限制性内切酶对193名平均年龄为(39.64±7.14)岁的健康女性和92名平均年龄为(43.96±6.59)岁的子宫肌瘤患者的ERβ基因扩增产物酶切,测定其基因多态性。结果ERβ的基因多态性AluI在子宫肌瘤组与对照组之间的分布分别为78.3%、19.6%、2.2%以及69.4%、29%、1.6%(P=0.229);RsaI在子宫肌瘤组与对照组之间的分布分别为65.2%、25%、9.8%以及67.4%、28.5%、4.1%(P=0.162),2种基因多态性的分布在2组之间差异均无统计学意义。结论在汉族人群中,ERβ基因的RsaI和AluI多态性分布与子宫肌瘤的发生无相关性。
Objective The present study was designed to investigate the possible association between estrogen receptor（ER） β gene polymorphisms and leiomyoma in a Chinese（Han） population.Methods The distribution of the ERβ gene polymorphisms were examined in 285 Chinese（Han） females： 92 patients and 193 healthy controls.Genomic DNA was obtained from whole blood.ERβ gene polymorphisms were assessed by the method of polymerase chain reaction-restriction fragment-length polymorphism（PCR-RFLP）.Results No significant differences between the leiomyoma patients and the controls in the frequencies of the polymorphisms in the ERβ gene were found in the subjects.Conclusion The RsaI and AluI polymorphisms of the ERβ gene were not associated with the establishment of leiomyoma of the uterus in the Chinese（Han） population.
出处
《首都医科大学学报》
CAS
北大核心
2010年第5期660-663,共4页
Journal of Capital Medical University
关键词
子宫肌瘤
基因多态性
ERΒ
leiomyoma of uterus
gene polymorphism
estrogen receptor β
