摘要
目的探讨hnRNPB1mRNA在非小细胞肺癌患者外周血中手术前后表达水平的变化及其临床意义。方法采用Realtime FQ-RT-PCR方法检测30例非小细胞肺癌患者手术前第3天和手术后第3、5、10天外周血中hnRNPB1mRNA的含量。结果非小细胞肺癌患者外周血中检测的hnRNPB1mRNA手术前第3天和手术后第5、10天的ΔΔCt值比较P为0.00,差异有统计学意义(P<0.05),手术前和手术后第3天的ΔΔCt值比较P为0.12,差异无统计学意义(P>0.05);手术前和手术后第3天鳞癌与其他病理类型肺癌ΔΔCt值比较P值分别为0.02和0.01,差异有统计学意义,而手术前后不同性别、不同病理分期和有无淋巴结转移组间以及手术后第5天和第10天不同病理类型的ΔΔCt值差异无统计学意义(P>0.05)。结论 hnRNPB1mRNA在非小细胞肺癌患者手术后外周血中的表达水平随时间推移逐渐下降,这种变化可以用来监测非小细胞肺癌患者的手术治疗效果和判断预后,并有可能用以术后监测肿瘤复发。另外hnRNPB1mRNA在非小细胞肺癌患者手术前和手术后早期外周血中的表达水平与病理类型有一定的关系。
Objective To investigate clinical significance of the expression of peripheral blood hnRNP B1（Heterogeneous nuclear ribonucleoprotein B1） mRNA in NSCLC patients.Method The peripheral blood hnRNP B1 mRNA was detected in 30 NSCLC patients at the third day pre-operation and third day,fifth day,tenth day after operation by real time FQ-RT-PCR.Results The ΔΔCt value of peripheral blood hnRNP B1 mRNA of third day before operation in NSCLC patients was significantly different from fifth and tenth day after operation（P0.05）,and the same as that of third day after operation.Meantime,there was no significant difference between the ΔΔCt value of fifth day and tenth day post-operation（P0.05）.The ΔΔCt values of squamous cell carcinoma at the third day before and after operation was significantly different from other pathological types.Whereas,the ΔΔCt vere not related with gender,pathological stage or lymph node metastasis.Conclusion The expression level of peripheral blood hnRNP B1 mRNA in NSCLC patients was gradually decreased after operation with a time-dependent manner,which was useful to monitor the therapeutic efficacy and evaluate prognosis or the relapse of tumor.Expression level of peripheral blood hnRNP B1 mRNA in NSCLC patients was related with pathological types.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第10期1152-1155,共4页
Cancer Research on Prevention and Treatment
关键词
非小细胞肺癌
核内不均一核糖核蛋白
外科治疗
Non small-cell lung cancer
Heterogeneity ribonucleoprotein
Surgical therapy
