摘要
目的:提高低温植酸酶产量。方法:通过单因素和正交实验研究温度、pH、发酵时间、接种量、装液量对菌株Y1的发酵影响。结果:最适发酵条件为温度15℃,pH5.0,发酵时间24h,接种量10%,装液量200ml/500ml。其最终酶活力达到了162.39U/mL。结论:经单因素和正交实验研究后其低温植酸酶酶活力比优化前提高了29.43%。
Objective：The purpose was to enhance the output of cold-active phytase.Method：The fermentation conditions of Y1 including temperature,pH,fermentation time,inoculum size and liquid volume in flask have been studied by single factor and orthogonal experiments.Result：The suitable liquid fermentation conditions were temperature 15℃,pH 5.0,fermentation time 24h,inoculum size 10%,liquid volume 250mL/500mL.Enzyme production eventually reached 162.39U/mL.Conclusion：The enzyme activity of cold-active phytase after fermentation conditions were optimized by single factor and orthogonal experiments was enhanced by 29.43% than that before optimization.
出处
《生物技术》
CAS
CSCD
北大核心
2010年第5期72-75,共4页
Biotechnology
基金
国家高技术研究发展计划"863"项目(2007AA021306)资助
关键词
低温植酸酶
发酵条件
正交实验
cold-active phytase
fermentation condition
orthogonal experiment
