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甘遂醇提物及不同极性溶剂萃取物对肿瘤细胞增殖的影响 被引量:4

Effects of the Extracts and Different Polar Parts of Kansui on Tumor Cells Proliferation
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摘要 目的观察甘遂醇提物及不同极性溶剂萃取物对体外培养MCF-7、A549、HepG2细胞生长的影响。方法采用甘遂醇提物及其环己烷、乙酸乙酯的萃取物和水溶性组分,分别与体外培养的MCF-7、A549、HepG2细胞作用48h,采用噻唑蓝(MTT)法测定各供试品对细胞增殖的影响。结果甘遂醇提物对MCF-7有较好的抑制作用,半数抑制浓度(IC50)为202μg·mL-1;甘遂乙酸乙酯萃取物和环己烷萃取物对MCF-7有较好的抑制作用,IC50分别为552和378μg·mL-1,仅在高浓度时对A549和HepG2细胞增殖有抑制作用;甘遂水溶性组分高浓度时对A549细胞和HepG2细胞增殖有促进作用。结论甘遂抑瘤成分可能存在于乙酸乙酯、环己烷萃取部位,促肿瘤生长成分可能存在于水溶性组分。 Objective To investigate the growth-modulating effects of the extracts and different polar parts of Kansui on the proliferation of MCF-7,A549,HepG2 cells.Methods The extracts of Kansui,acetate extract,cyclohexane extract and water extract from Kansui were co-cultured with MCF-7,A549,and HepG2 cell lines,and the cell proliferation was evaluated by MTT colorimetric assay.Results The ethanol extract of Kansui obviously reduced MCF-7 proliferation,with IC50 values as 202 μg·mL-1;Acetate extract and cyclohexane extract from Kansui suppressed MCF-7 as well,with IC50 as 552 μg·mL-1 and 378 μg·mL-1,respectively.They stimulated the growth of A549 and HepG2 cells at a high concentration.The water soluble parts from Kansui stimulated A549 and HepG2 cells proliferation at high levels.Conclusion The anticancer components in Kansui could be exist in acetate extracts and cyclohexane extracts,while the cancer-stimulating compotents be in the water extracts from Kansui.
出处 《医药导报》 CAS 2010年第11期1416-1418,共3页 Herald of Medicine
基金 2009年湖北省教育厅中青年人才项目(基金编号:Q20091601)
关键词 甘遂 醇提取物 萃取物 抗肿瘤作用 Kansui Acetate extract Cyclohexane extract Anticancer effect
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  • 1梁爱华,聂淑琴,薛宝云,李桂琴,李泽琳.炮制对苦杏仁特殊毒性及药效的影响[J].中国中药杂志,1993,18(8):474-478. 被引量:11
  • 2聂淑琴,薛宝云,梁爱华,李桂琴,李泽琳.炮制对牛膝特殊毒性的影响[J].中国中药杂志,1995,20(5):275-278. 被引量:7
  • 3李忌,郑耘,郑荣梁,贾忠健.天然二萜类化合物的抗肿瘤活性[J].肿瘤防治研究,1995,22(5):271-272. 被引量:12
  • 4任毅,金玉姬,胡正海.甘遂块根的发育解剖学研究[J].西北植物学报,1995,15(2):104-109. 被引量:9
  • 5马金双,吴征镒.中国大戟属的修订[J].云南植物研究,1995,17(3):291-295. 被引量:3
  • 6HAN X Y,SUN B Q,WU A X,ZHANG J Y. Effects of Euphorbia kansui on antifertility[J]. J. Cina Modern Medicine, 1993,3(4) : 21 - 23.
  • 7ZHENG W F,CUI Z,ZHU Q. Cytotoxicity and antiviral activity of the compounds from Euphorbia kansui[J]. J. Planta Medica, 1998, 64:754- 757.
  • 8WANG L Y,WANG L N,YAO X S,et al. Diterpenes from the roots of Euphorbia kansui and their in vitro effects on the cell division of xenopus(2) [J]. Chem. Pharm. Bull. , 2003,51(8) : 935- 941.
  • 9YASUKAWA K, AKIHISA T,YOSHIDA Z Y,et al. Inhibitory effect of euphol,a triterpene alcohol from the roots of Euphorbia kansui, on tumour promotion by 12-o-tetradecanoylphorbol-13-acetate in two-stage carcinogenesis in mouse skin[J]. J. Pharm. Pharmacol. ,2000, 52(1) :119-124.
  • 10王文采.华西南大戟科植物小志[J]云南植物研究,1988(01).

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