摘要
以野生红蘑为原料,复合酶法浸提红蘑中真菌多糖。在单因素试验的基础上,采用正交试验设计获得最佳多糖提取工艺,即酶解温度60℃、纤维素酶和木瓜蛋白酶的比例为3:1(m/m)、pH5.0、酶解3h,此条件下提取多糖得率为12.0%。与传统方法相比,该工艺具有提取条件温和,提取时间较短,对多糖损伤小等特点。
For polysaccharide extraction,wild Gomphidius rutilus fruit bodies were hydrolyzed with cellulase and papain together.Based on single factor investigations,orthogonal array design was employed to optimize the hydrolysis process,and the optimum hydrolysis conditions were found as follows：hydrolysis temperature 60 ℃;cellulase/papain mass ratio 3：1;pH 5.0;and hydrolysis duration 3 h and the polysaccharide yield obtained under such conditions was 12.0%.This method has many advantages over conventional methods,such as gentle reaction conditions,time saving and less destructive effect on polysaccharide structure.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第18期143-146,共4页
Food Science
基金
鞍山科技计划项目(08SF24)
关键词
红蘑
多糖
提取
复合酶
Gomphidius rutilus
polysaccharide
extraction
compound enzymes
