摘要
以普通茶籽为研究对象,用还原能力来表示茶籽水提物的抗氧化能力。在单因素试验基础上,选取液料比、提取时间、提取温度为自变量,还原能力(吸光度)为响应值,利用Box-Benhnken中心组合设计原理和响应面分析法,研究各自变量及其交互作用对还原能力的影响,模拟得到二次多项式回归方程的预测模型,并确定最佳提取条件为液料比16:1(mL/g)、提取时间1.6h、提取温度81℃。在此条件下,还原能力(吸光度)为0.616±0.005,与预测值0.610较为一致。
Camellia sinensis seed powder was extracted with hot water to obtain bioactive compounds with reducing power and three extraction parameters including liquid/material ratio,length of extraction time and extraction temperature were optimized using central composite design and response surface methodology based on single factor investigations for achieving maximum reducing power（reflected by 700 nm absorbance of the reaction system）.A mathematical quadratic polynomial regression equation reflecting the relationship between extract reducing power and the above extraction parameters was set up.The optimal extraction parameters were found as follows：liquid/material ratio（mL/g） 16：1;extraction temperature 81 ℃;and length of extraction time 1.6 h.Under the optimized conditions,the actual 700 nm absorbance of reducing power was 0.616 ± 0.005,close to the predicted value of 0.610.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第18期170-174,共5页
Food Science
关键词
普通茶籽
抗氧化
还原能力
响应面分析法
Camellia sinensis seeds
antioxidation
reducing power
response surface methodology
