摘要
给ＳＤ大鼠腹腔注射氯化铝溶液６０天，研究不同剂量铝对神经行为以及各脑区神经细胞数和海马颗粒空泡变性（ＧＶＤ）的影响。穿梭箱实验和敞箱行为实验表明，按体重腹腔注射４．０ｍｇ／ｋｇ和１０．０ｍｇ／ｋｇ可使大鼠主动回避反应能力和自发活动显著下降。光镜下对ＨＥ染色的大脑顶叶、小脑和海马切片进行神经细胞计数，发现腹腔注射１０．０ｍｇ／ｋｇ可使大脑顶叶椎体层椎体细胞、海马椎体细胞数和小脑浦肯野细胞数显著减少。光镜下发现各组海马神经细胞均发生ＧＶＤ，腹腔注射４．０ｍｇ／ｋｇ和１０．０ｍｇ／ｋｇ组ＧＶＤ细胞数有明显增加；各组ＧＶＤ细胞阳性率与铝剂量有显著相关性。本结果进一步证实了铝的神经行为毒性，并提示该毒性与铝导致神经细胞数减少有关。
Effect of aluminum on neurobehavior and the number of neurons in different domains of brain as well as granulovacuolar degeneration(GVD) in hippocampus were observed in Sprague Dawley rats by intraperitoneal injection with aluminum choride for 60d. The injection of aluminum at the dosage of 4 0 or 10 0mg/kg could decrease active aviodance response and spontaneous motor activity in the shuttle box test and the open field test significantly. Sections from cerebrum, cerebellum and hippocampus were detected with microscope after HE staining.Aluminum(10 0 mg/kg) could result in the decrease of neurons in cerebrum, Pukinje cells in cerebellum and pyramidal neurons in hippocampus. GVD of nerve cells in hippocampus were observed in each group. while the dosages of Al injected was 4 0 and 10 0 mg/kg, the number of GVD cells increased significantly. The incidence of GVD per 300 nerve cells was significantly related to the dosage of aluminum.
出处
《卫生研究》
CAS
CSCD
北大核心
1999年第3期164-166,共3页
Journal of Hygiene Research
关键词
铝
学习记忆
神经细胞
颗粒空泡变性
神经毒性
Aluminum,\ Learning memory,\ Nerve cells,\ Granulovacuolar degeneration
