摘要
通过单因素和正交试验对双孢蘑菇AS2796的培养条件和培养基进行了优化,优化后的培养基为蔗糖15.0g/L,玉米淀粉30.0g/L,蛋白胨2.0g/L,黄豆粉30.0g/L,MgSO4.7H2O 1.0g/L,KH2PO4 1.5g/L;培养条件:初始pH值为6.5,发酵温度25℃,时间7d,摇床转速180r/min,装液量50mL,接种量5%(v/v)。
Composition of medium and fermentation conditions of Agaricus bisporus AS2796 in liquid culture were optimized with one factor and orthogonal test methods. The results showed that the optimum medium was （g/L）： sucrose 15.0, corn starch 30.0, peptone 2.0, soybean powder 30.0, MgSO4·7H2O 1.0, KH2PO4 1.5 and the optimum fermentation condition were initial pH value 6.5, fermentation under 25℃ for 7d, rotation speed 180r/min with broth volume 50ml, inoculum 5%.
出处
《中国酿造》
CAS
北大核心
2010年第11期129-131,共3页
China Brewing
基金
天津市科技计划项目(08ZCZDNC04600)
天津市科技计划项目(08ZHNZNC03600)
