ATP后适应对大鼠心肌缺血再灌注损伤的保护作用被引量：1

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摘要目的观察三磷酸腺苷(ATP)后适应及ATP后适应不同给药方式对大鼠在体心肌缺血再灌注损伤(MI-RI)的影响。方法采用结扎大鼠左冠状动脉前降支30 min、再灌注120 min的方法复制大鼠心肌MIRI模型。实验动物随机分为假手术组、缺血再灌注组、ATP后适应组,ATP后适应+再灌注早期给药组。实验终点测定心肌梗死面积,观察血清丙二醛(MDA)及总超氧化物歧化酶(T-SOD)含量变化,应用免疫组化法测定组织中NF-κB的表达,TUNEL法检测心肌细胞凋亡指数。结果与缺血再灌注组相比,ATP后适应组心肌梗死面积明显缩小,血清MDA含量降低,NF-κB表达降低,心肌细胞凋亡指数降低,血清T-SOD含量升高(P<0.05或<0.01);ATP后适应组和ATP后适应+再灌注早期给药组的各项指标无统计学差异(P>0.05)。结论 ATP后适应对心肌MIRI有保护作用,其机制可能与抗氧自由基损伤、抑制缺血再灌注后NF-κB过度表达及减少心肌细胞凋亡有关。

作者姚丹刘仁光

机构地区辽宁医学院附属第一医院心血管病研究所内蒙古玛拉沁医院

出处《山东医药》 CAS 北大核心 2010年第41期38-39,共2页 Shandong Medical Journal

关键词三磷酸腺苷后适应心肌再灌注损伤细胞凋亡大鼠

分类号 R542.2 [医药卫生—心血管疾病]

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参考文献6

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