摘要
目的：探讨细胞周期蛋白Ｄ１（ｃｙｃｌｉｎＤ１）的表达与喉鳞癌（ＳＣＣＬ）的关系。方法：对３５例经病理证实ＳＣＣＬ患者的肿瘤组织及其邻近的正常粘膜，运用反转录多聚酶链反应（ＲＴ－ＰＣＲ）、光密度半定量法，分别检测其ｃｙｃｌｉｎＤ１的表达程度。结果：发现几乎所有的肿瘤标本的的表达量要高于正常粘膜（Ｐ＜０．０５），有淋巴结转移者的表达量要明显高于无淋巴结转移者（Ｐ＜０．０５）；表达量与ＴＭＮ分期也有明显的相关性（Ｐ＜０．０１）；但与病理分级无关（Ｐ＞０．０５）。在性别和年龄分布上表达的程度也无差异。结论：ｃｙｃｌｉｎＤ１表达程度的检测技术可能成为判断ＳＣＣＬ患者的治疗效果及预后估计的提示性依据。
Objective: To study the correlation between the
expression level of Cyclin D1 and the laryngeal squamous cell carcinoma (LSCC). Method: 35
LSCC cases testified pathologically were included in the study.The samples obtained from each
tumor tissue and adjacent normal mucosa were detected to see the expression level of Cyclin
D1 with the method of RTPCR and light density half quantive analysis. Result: The expression
level of Cyclin D1 was significant higher in almost all turmor tissues than in the adjacent
normal mucosae(P<0.05).Overexpressed carcinomas were more frequently associated with
lymph node metastases(P<0.05).A significant association between the expression level of
Cyclin D1 and TNM was found.The higher T stage,the higher the expression level of Cyclin
D1(P<0.01).The expression level of low histological grade carcinomas was lower than that of
the high histological grade carcinomas,but the difference had no statistical significance.No
association between overexpression of Cyclin D1 and sex,age was found too. Couclusion: The
result indicats that the expression level of Cyclin D1 in LSCC may be a useful marker of
prognostic significance and it provide a molecular biology basis for treating the LSCC patients.
出处
《临床耳鼻咽喉科杂志》
CSCD
1999年第6期268-270,共3页
Journal of Clinical Otorhinolaryngology
基金
上海市跨世纪百人培养计划课题
