摘要
目的探讨新疆吐鲁番地区维吾尔族2型糖尿病(T2DM)高血压与HLA-DQA1等位基因的相关性。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)技术,对新疆吐鲁番地区维吾尔族65例正常对照者、42例高血压患者、73例T2DM患者和37例T2DM高血压患者的HLA-DQA1等位基因频率进行检测,分析T2DM高血压HLA-DQA1等位基因多态性。结果与正常对照组比较,T2DM组HLA-DQA1*0104等位基因频率增高;T2DM高血压组HLA-DQA1*0104、HLA-DQA1*0301、HLA-DQA1*0501等位基因频率增高,差异均有统计学意义(P<0.05)。与高血压组比较,T2DM高血压组HLA-DQA1*0104等位基因频率明显增高,差异有统计学意义(P<0.01)。结论 HLA-DQA1*0104可能是新疆吐鲁番地区维吾尔族T2DM的易感基因;HLA-DQA1*0104、HLA-DQA1*0301、HLA-DQA1*0501可能是新疆吐鲁番地区维吾尔族T2DM高血压的易感基因。
Objective To investigate the association between the polymorphism of HLA-DQA1 alleles and type 2 diabetes hypertention in Uygur nationality of Xinjiang Turpan.Methods Polymerase chain reaction with sequence-specific primers （PCR-SSP）genotyping method was conducted in 65 Uygur healthy individuals,42 patients with hypertention,73 patients with type 2 diabetes mellitus and 37 patients with type 2 diabetes hypertention from Xinjiang Turpan.Then a study was made on the association between the polymorphism of HLA-DQA1 alleles and type 2 diabetes hypertension.Results Compared with normal control group,the frenquency of HLA-DQA1＊0104 allele in the patients with type 2 diabetes mellitus was higher;the frenquencies of HLA-DQA1＊0104,HLA-DQA1＊0301,HLA-DQA1＊0501 alleles in the patients with type 2 diabetes hypertension were higher.Compared with hypertension group,the frenquency of HLA-DQA1＊0104 allele in the patients with type 2 diabetes hypertension were higher.Conclusion In Uygur nationality of Xinjiang Turpan,HLA-DQA1＊0104 allele is susceptible gene of type 2 diabetes;HLA-DQA1＊0104,HLA-DQA1＊0301,HLA-DQA1＊0501 alleles are susceptible genes of type 2 diabetes hypertension.
出处
《新疆医科大学学报》
CAS
2010年第8期880-882,885,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金项目(30660074)
