摘要
克鲁维酵母（Ｋｌｕｙｖｅｒｏｍｙｃｅｓｓｐ．）Ｙ－８５产生的胞内菊粉酶（ｅｎｄｏｃｅｌｕｌａｒｉｎｕｌｉｎａｓｅ），以Ｄ２０１－ＧＭ大孔径阴离子交换树脂吸附交联法固定化，其制备固定化酶（ＩＥ）的适宜条件：树脂吸附酶时ｐＨ６．５、温度３０℃、时间３ｈ；交联时戊二醛浓度０．０３％、温度４℃、时间３ｈ。上述条件下制得ＩＥ的活性产率可达６２％，水解菊粉底物的最适温度５５℃，对热的稳定性和贮存稳定性均有明显提高，用ＩＥ填充床反应器连续降解菊粉抽提液（总糖４．５％）的实验结果表明，进料空间速度（ＳＶ）１．７ｈ－１，５０℃下操作，菊粉水解率达１００％，容器定容产率（ＱＰ）７７ｇ·Ｌ－１·ｈ－１，ＩＥ半衰期２７ｄ。
The partially purified endocellular inulinase from Xluyve romyces sp.Y 85 was immobilized by adsorb crosslinking method on D 201 GM macroporous resin.The conditions of the immobilized enzyme preparation was investigated.Under suitable conditions,the prepared immobilized inulinase had ac tivity yield of 62%.The optimum temperature for inulin hydrolysis by the immobil ized inulinase was 55℃.Thermal and storage stability of the enzyme were improve d by immobilization.In an immobilized inulinase packed bed reactor,4.5%inulin s olution of Jerusalem artichok extract was completely hydrolyzed at 50℃ and space velocity of 1.7h -1 ,the volumetric productivity was 77g·L -1 ·h -1 ,the half life of immobilized inulinase at 50℃ was 27 days.
出处
《工业微生物》
CAS
CSCD
1999年第2期10-15,共6页
Industrial Microbiology
基金
国家科委"八.五"攻关课题
关键词
树脂
菊粉酶
吸附交联
固定化
Endocellular inulinase
adsorb crosslinking
immobil ization
properties.
