摘要
叶肉亲本（粗柠檬）型植株叶形指数、气孔特征与亲本粗柠檬无异，与同组合体细胞杂种差异显著。染色体计数为二倍体（2n=2x=18）。过氧化物酶（POX）、多酚氧化酶（PPO）、谷草转氨酶（GOT）同工酶图谱与粗柠檬一致。RAPD分析表明，在具多态性的54个随机引物中，大多数引物（52个）上的图谱与粗柠檬相同。但OPW－12上，叶肉亲本型植株含有哈姆林甜橙的特征谱带。OPV－04扩增产物显示，叶肉亲本型植株间存在细微差别。说明叶肉亲本型植株的遗传物质绝大多数来自叶肉亲本粗柠檬，少量来源于胚性悬浮系亲本哈姆林甜橙。
The leaf indexes and stoma characteristics of leaf-parent-type regenerants were the same as those of the leaf parent Rough lemon, but quite different from the somatic hybrids. Leaf-Parent-type plants were diploid (2n=2x=18). Patterns of POX, PPO, GOT isozymes were identical to those of Rough lemon. The plants were analyzed by using 54 arbitrary primers with polymorphisms. Most of the primers (52) showed that leaf-parent-type plants were in concordance with Rough lemon. However, with primer OPW-12, the plants contained a unique band of Hamlin sweet orange. Amplification products with primer OPV-04 showed slight differences among the accessions. The research suggested that most genetic constitutions of the leaf-parent-type plants were from leaf parent Rough lemon and a little from embryogenic suspension parent Hamlin sweet orange.
基金
国家自然科学基金!39330150
关键词
柑桔
体细胞融合
再生植株
RAPD
Citrus, Somatic hybridization, Plant regeneration, Random amplified polymorphic DNA (RAPD)
