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JC病毒衣壳蛋白VP1多克隆抗体的制备 被引量：1

Preparation of JC virus capsid protein-VP1 polyclonal antibody
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摘要 目的制备pET-32a(+)-VP1蛋白的多克隆抗体。方法用纯化后的VP1蛋白分4次免疫兔子,颈动脉插管法取血,制得多克隆抗体。结果用ELISA法和Western blot鉴定多克隆抗体的效价得1?320000。该抗体可以用Western blot法检测出59 kD左右的VP1蛋白。结论成功制备高效价的JC病毒衣壳蛋白VP1多克隆抗体。 Objective To preparate the pET-32a（＋）-VP1 polyclonal antibody.Method Rabbits were immuned with purified VP1 four times.Polyclonal antibody was made by carotid artery intubation and withdrawing blood.Result The titer of polyclonal antibody was 1︰320000 detected by ELISA and Western blot.VP1 protein with the molecular weight at approximate 59 kD had been detected by Western blot.Conclusion The study successfully prepare JC virus capsid protein-VP1 polyclonal antibody with high titer.
作者 黄敏 杨琼 李炜 王琦 成军
机构地区 大连医科大学微生物教研室 北京地坛医院传染病研究所
出处 《中国微生态学杂志》 CAS CSCD 2010年第10期884-885,893,共3页 Chinese Journal of Microecology
基金 北京市科委资助项目(D0906003040291)
关键词 JC病毒 VP1蛋白 多克隆抗体 JC virus； VP1 protein； Polyclonal antibody；
分类号 R446.62 [医药卫生—诊断学]
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参考文献8

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共引文献2

同被引文献12

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中国微生态学杂志
2010年 第10期

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