摘要
采用氢氧化钾-无水乙醇溶液直接皂化,石油醚萃取,液相色谱法测定乳制品中的胆固醇和维生素A(VA),讨论并确定了乳制品中样品皂化、萃取的最佳条件。胆固醇和维生素A的回收率分别为96.56%～106.09%和92.50%～112.00%,RSD分别为2.71%～4.33%和2.98%～4.38%(n=11)。该法具有简便、快速、灵敏、准确等优点。
A simple and rapid method has been developed for simultaneous determination of cholesterol and Vitamin A in milk product. The method involves alcoholic KOH saponification of the samples, extraction with petroleum ether, and determination by liquid chromatography. The recoveries of cholesterol and VA in milk product were 90.00%～105.00% and 92.50%～112.00%, with RSDs of 2.71% ～4.33% and 2.98%～4.38%respectively.
出处
《科技信息》
2010年第22期14-15,共2页
Science & Technology Information
基金
南方医科大学公共卫生与热带医学学院院长基金项目(2009年)
关键词
高效液相色谱法
胆固醇
维生素A
乳制品
High performance liquid chromatography cholesterol vitamin A milk product
