摘要
目的:研究炮制对苦杏仁中主要成分苦杏仁苷在大鼠体内代谢的影响。方法:分别给SD大鼠注射和灌胃苦杏仁苷,灌胃苦杏仁生品及炮制品后,于不同时间点采血,高效液相色谱-质谱联用法分析血浆中苦杏仁苷及其代谢产物,绘制药时曲线。结果:苦杏仁苷注射给药后在血浆中以原型形式存在,而苦杏仁苷、苦杏仁生品和炮制品灌胃给药后均未检测到原型,其代谢产物经质谱鉴定为野樱苷;且炮制后代谢产物野樱苷在大鼠体内的药时曲线与生品有明显不同。结论:炮制对苦杏仁在大鼠体内的代谢过程有较大影响。
Objective： To study the influence of processing on metabolism of the main component of bitter almond-amygdalin in rat. Method： The blood was collected at different times after amygdalin given by injection and oral, bitter almond and its processed production given by oral respectively, and then detected by both HPLC and HPLC-MSn methods after extraction pretreatment. Result： After injection, amygdalin was absorbed in prototype to blood rapidly, while the other three kinds of medicine given by oral were all not detected the prototype of amygdalin, but two metabolites were detected which were isomers of prunasin confirmed by mass spectrometry. The metabolic pathway of prunasin in processed bitter almond group was markedly different from the bitter almond group. Conclusion： Processing has a significant effect on bitter almond metabolic processes in rats.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第20期2684-2688,共5页
China Journal of Chinese Materia Medica
基金
陕西省中医管理局基金项目(陕中医发20072506
20072507)
