摘要
【研究目的】利用流式细胞术、DTH法和LDH法探讨葱乙醇提取物对小鼠特异性和非特异性免疫的影响,为调节动物免疫状态和改善机体防御能力提供试验依据。【方法】将60只昆明种小鼠随机分为环磷酰胺组(C)、环磷酰胺+葱乙醇提取物组(C+A)、葱乙醇提取物组(A)和生理盐水组(S)。环磷酰胺皮下注射造成小鼠免疫抑制;C+A组和A组给小鼠葱乙醇提取物连续灌胃35天;S组灌服等量生理盐水。观察测定临床症状、免疫器官指数、细胞免疫功能、体液免疫功能和非特异性免疫功能。【结果】葱乙醇提取物可以提高免疫器官指数;对免疫抑制小鼠的左右耳重量差、CD4+/CD8+值、血清抗体和巨噬细胞吞噬活性都有促进作用,对正常小鼠的腹腔巨噬细胞吞噬活性和NK细胞活性有促进作用。【结论】葱乙醇提取物对小鼠的特异性免疫和非特异性免疫功能具有显著的调节作用。
[OBJECTIVE]To investigate the influence of ethanol extract of the allium in mouse-specific and non-specific immunity by DTH method,flow cytometry and HC50 assay ,and provide the basic evidence for the regulation of the immune status of animals and improvement of the body＇s defense.[METHOD]60 Runming mice were randomLy divided into the cyclophosphamide group （C）,cyclophosphamide ＋ alcoholic extract group （C ＋ A）,ethanol extract of the allium group （A） and normal saline group （S）. Subcutaneous injection of immune suppression caused by cyclophosphamide in mice; C ＋ A group and A group of mice were fed ethanol extract of the allium consecutive 35 days; S group fed with normal saline. A series of tests were carried out such as clinical symptoms,the immune organ index,the cellular and humoral immune function and the non-specific immune function.[RESULTS]The results showed that ethanol extract of the allium enhanced the immune organ index; increased the weight difference of the left and right ear,CD4 ＋/CD8 ＋ values,serum antibody and phagocytic activity in immune suppression mice. The ethanol extract of the allium promoted phagocytic activity and NK cell activity in the normal mice.[CONCLUSION]Ethanol extract of the allium could significantly regulate the specific immunity and non-specific immunity function in mice.
出处
《中国农学通报》
CSCD
北大核心
2010年第20期7-10,共4页
Chinese Agricultural Science Bulletin
基金
河北省科技支撑计划项目"高通量筛选抗病毒中兽药活性成分样品库建立及禽类重大传染病的防控"(07220401D-01)
河北省自然科学基金项目"白头翁素对腹泻仔猪肠道粘膜修复基因EGFR
TGFα表达的影响"(C2008000334)
关键词
葱乙醇提取物
小鼠
环磷酰胺
特异性免疫
非特异性免疫
ethanol extract of the allium； mouse； cyclophosphamide； specific immunity； non-specific immune；
