摘要
目的 研究重复经颅磁刺激对血管性痴呆（VD）大鼠认知功能康复的影响,探讨其康复治疗的分子学机制.方法 将36只Wistar大鼠随机分为正常对照组、VD模型组、VD低频组和VD高频组,VD低频组和VD高频组大鼠接受的刺激频率分别为0.5 Hz和5 Hz.采用Morris水迷宫测试方法检测各组大鼠认知功能,透射电镜下观察各组大鼠海马CA1区超微结构变化.应用实时定量逆转录多聚酶链反应（RT-PCR）、Western blot方法分别检测海马突触素、脑源性神经营养因子、N-甲基-D-天冬氨酸受体1的mRNA及蛋白表达.结果 大鼠Morris水迷宫测试结果提示2个治疗组认知功能有改善,各项指标均优于VD模型组（P＜0.05）;透射电镜观察结果从形态学上显示2个治疗组突触界面曲率、突触后致密物质厚度、突触活性带长度均较VD模型组增加（P＜0.05）.2个治疗组各检测因子表达量也明显高于VD模型组（P＜0.05）.结论 重复经颅磁刺激对VD致认知功能障碍有治疗作用,其机制可能与其促进海马突触素、脑源性神经营养因子、N-甲基-D-天冬氨酸受体1的表达有关.
Objectiye To study the mechanism by which transcranial magnetic stimulation （rTMS） affects cognitive dysfunction in vascular dementia （VD）. Methods Thirty-six male Wistar rats were randomly divided into a control group, a VD group, a low frequency rTMS group and a high frequency rTMS group. Two-vessel occlusion was employed to induce VD models. Low frequency rTMS group rats were given 0.5 Hz rTMS for six weeks. High frequency rTMS group rats were given 5 Hz rTMS for six weeks. Morris＇ water maze test was used to measure their spatial learning ability and memory. The ultrastructures of the synapses in the four groups were detected with transmission electron microscopy. The expression of synaptophysin （SYN）, brain derived neurotrophic factor （BDNF） and Nmethyl-D-aspartate receptor 1 （ NMDAR1 ） mRNA and protein in the hippocampus were determined by real-time PCR and Western blotting. Results The behavior and morphology of the rats treated with rTMS improved. The average expression of SYN, BDNF and NMDAR1 mRNA and protein in the low frequency rTMS group and the high frequency rTMS group were significantly higher than in the VD group. Conclusion rTMS can provide a rehabilitative effect for VD. The mechanism might be associated with enhancing the expression of SYN, BDNF and NMDAR1 in the hippocampus.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2010年第10期730-734,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
天津市科技发展计划重点项目（06YFSZSF01700）
