期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

氟化钠对人黄韧带细胞碱性磷酸酶活性及骨钙素合成的影响 被引量：1

Influence of sodium fluoride on alkaline phosphatase activity and bone gla protein synthesis in human yellow ligament cells in vitro
原文传递
导出
摘要 目的 探讨氟化钠（NaF）对体外培养条件下人黄韧带细胞碱性磷酸酶（alkaline phosphatase,ALP）活性及骨钙素（bone gla protein,BGP）合成的影响.方法 依据手术标本来源不同,分为正常黄韧带细胞（NLF）组（取自急性外伤性胸腰椎骨折截瘫患者,7例）、退变黄韧带细胞（DLF）组（取自退变性腰椎管狭窄症患者,9例）及骨化黄韧带细胞（OLF）组（取自胸椎黄韧带骨化患者,8例）.采用组织块贴壁法进行体外细胞培养,共获得24组传代细胞.取第五代细胞加入不同浓度的NaF,观察细胞形态变化,并测定NaF对各组人黄韧带细胞的ALP活性和BGP合成的影响.结果 不同来源的人黄韧带细胞均可在体外增殖并传代,DLF与OLF组人黄韧带细胞呈多形性表现,并可形成钙结节.体外培养条件下,高浓度（1.0mmol/L）NaF可导致人黄韧带细胞发生中毒反应；低浓度（0.01～0.125 mmol/L）NaF可促进DLF组黄韧带细胞增殖、钙结节形成,ALP活性上调,BGP合成明显增加,而对OLF与NLF组人黄韧带细胞则无明显效应.结论 体外培养条件下,低浓度（0.01～0.125 mmol/L）NaF可促进人退变黄韧带细胞的ALP活性增高及BGP合成增加,提示低浓度NaF可能促进人退变黄韧带细胞向成骨方向分化. Objective To investigate the influence of sodium fluoride（NaF）on alkaline phosphatase （ALP）activity and bone gla protein（BGP）synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells were divided into three groups according to its sources,including normal yellow ligament cells（NLF）group（from acute traumatic thoracolumbar fractures with paraplegia in 7 patients）,degenerative yellow ligament cells（DLF）group（from degenerative lumbar stenosis in 9 cases）and ossified ligament cells（OLF）group（thoracic yellow ligament from 8 patients）.Twenty-four groups of cells were obtained under vitro cell culture by the method of tissue adherence.Different concentrations of NaF were added into the medium when the cells spread to the fifth generation.Then,the morphological changes were observed and ALP activity and BGP synthesis were tested.Results Human yellow ligament cells from different samples can proliferate and be passaged in vitro.The cell in ossific groups and degenerative groups were pleomorphic and could form calcium nodules.High concentration of NaF（1.0 mmol/L）can lead to cytotoxic reaction in all 24 groups.Low concentrations of（0.01-0.125 mmol/L）NaF can enhance the ALP activity and BGP synthesis in DLF groups while no effect was found in OLF and NLF groups cells under the same concentration of NaF.Conclusion The fact that fluoride can promote ALP activity and BGP synthesis in degenerative yellow ligament cells in vitro indicates fluoride may play an important role in inducing further ossification of human ligament cells.
作者 王哲 胡学昱 罗卓荆 杜俊杰 李新奎 王全平
机构地区 第四军医大学西京医院脊柱外科
出处 《中华骨科杂志》 CAS CSCD 北大核心 2010年第11期1138-1143,共6页 Chinese Journal of Orthopaedics
关键词 黄韧带 氟化钠 碱性磷酸酶 骨钙素 Ligamentum flavum Sodium fluoride Alkaline phosphatase Osteocalcin
分类号 R686 [医药卫生—骨科学]
  • 相关文献

参考文献16

  • 1Wang W,Kong L,Zhao H,et al.Thoracic ossification of ligamentum flavum caused by skeletal fluorosis.Eur Spine J,2007,16(8):1119-1128.
  • 2Wang W,Kong L,Zhao H,et al.Ossification of the transverse atlantal ligament associated with fluorosis:a report of two cases and review of the literature.Spine(Phila Pa 1976),2004,29(4):E75-78.
  • 3Muthukumar N.Ossification of the ligamentum flavum as a result of fluorosis causing myelopathy:report of two cases.Neurosurgery,2005,56(3):E622.
  • 4Suyama T,Hayashi K.Experimental studies on the ossification of the posterior longitudinal ligament of the cervical spine.Nippon Seikeigeka Gakkai Zasshi,1986,60(5):523-528.
  • 5范东伟,陈仲强.胸椎黄韧带骨化发病机制的研究进展[J].中华骨科杂志,2006,26(3):211-214. 被引量：12
  • 6王哲,张俊华,王全平,吕荣.胸椎黄韧带骨化与腰椎黄韧带退变的组织病理与超微结构比较研究[J].第四军医大学学报,2002,23(2):177-179. 被引量：7
  • 7Yayama T,Uchida K,Kobayashi S,et al.Thoracic ossification of the human ligamentum flavum:histopathological and immunohistochemical findings around the ossified lesion.J Neurosurg Spine,2007,7(2):184-193.
  • 8Wang Z,Li XD,Li MQ,et al.Changes in basic metabolic elements associated with the degeneration and ossification of ligamenta flava.J Spinal Cord Med,2008,31(3):279-284.
  • 9Vanden Bossche L,Vanderstraeten G.Heterotopic ossification:a review.J Rehabil Med,2005,37(3):129-136.
  • 10Ohgushi H,Dohi Y,Katuda T,et al.In vitro bone formation by rat marrow cell culture.J Biomed Mater Res,1996,32(3):333-340.

二级参考文献33

  • 1倪斌,贾连顺,戴力扬,刘洪奎,侯铁胜,赵定麟.胸椎黄韧带骨化所致椎管狭窄症的诊断及手术治疗[J].中国脊柱脊髓杂志,1994,4(2):56-59. 被引量：22
  • 2Kon T,Yamazaki M,Tagawa M,et al.Bone morphorgenetic protein-2 stimulates differentiation of cultured spinal ligament cells from patients with ossification of the posterior longitudinal ligament[J].Calcif Tissue Int, 1997,60(1):291-296.
  • 3Ishida Y,Kawai SH.Effects of bone-seeking hormones on DNA synthesis,cyclic AMP level,and alkaline phosphatase activity in cultured cells fron human posterior longitudinal ligament of the spine[J].J Bone Miner Res, 1993,8(11):1291-1300.
  • 4Rodan GA, Rodan SB. Expression of the Osteoblastic Phenoptype[M].In:pecK WA (ed).Bone and Mineral Rresearch. Amsterdam:Annual Z Eelsevier, 1982.244-285.
  • 5Yoshida M,Shima K,Taniguchi Y,et al. Hypertrophied ligament flavum in lumbar spinal cannal stenosis [J].Spine, 1992,17(11 ):1353-1360.
  • 6Maniatopoulos C,Sodek J,Melcher AH. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats[J].Cell Tissue Res, 1988,254(3):317-330.
  • 7Manduca P,Sanguineti C,Pistone M,et al. Differential expression of alkaline phosphatase in clones of human osteoblast-like cells[J].J Bone Miner Res,1993,8(2):251-300.
  • 8Chavassieux PM, Chenu C,Valentin-Opran A,et al.Influence of experimental eonditons on osteoblast activity in human primary bone cell cultrues[J].J Bone Miner Res, 1990,5:337-343.
  • 9Specchia N,Pagnotta A,Gigante A,et al. Characterization of cultured human ligamentum flavum cells in lumbar spine stenosis[J].J Orthop Res,2001,19(2):294-300.
  • 10Hayashi K, Ishidou Y,Yonemori K.Expression and location of bone morphorgenetic proteins (BMPs) and BMP receptors in ossification of the ligamentum flavum[J].Bone,1997,21 (1):23-30.

共引文献24

同被引文献21

  • 1Miyazaki M, Tsumura H, Wang JC, et al. An update on bone substitutes for spinal fusion. Eur Spine J.2009;18(6 ):783-799.
  • 2Ganey T, Libera J, Moos V, et al. Disc chondrocyte transplantation in acanine model : atreatment for degenerated damaged intervertebral disc.Spine.2003;28 (23):2609- 2620.
  • 3Zhang Y, Drapeau S,Howard SA,et aI.Transplantion of goat bone marrow stromal cells to the degenerating intervetebral disc in a goat disc-injury model. Spine.2011 ;36(5):372-377.
  • 4Wang Yr, Wu XT, Wang F. regeneration potential and mechanism of bone marrow mesenchymal stem cell transplantation for treating intervertebral disc degeneration.J Orthop Sci.2010;15(6): 707-719.
  • 5Choi ST, Kim JH, Kang EJ,et al.Osteopontin might be involved in bone remodelling rather than in inflammation in ankylosing spondylitis. Rheumatology(Oxford).2008; 47(12) 1775 -1779.
  • 6Bobacz K, Ullrich R, Amoyo L, et al. Stimulatory effects of distinct members of the bone morphogenetic protein family on ligament fibroblasts. Ann Rheum Dis. 2006;65(2):169-177.
  • 7Samee M, Kasugai S, Kondo H, et al. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) transfection to human periosteal cells enhances osteoblast differentiation and bone formation. Pharmacol Sci. 2008; 108(1):18-31.
  • 8Yu YY, Lieu S,Lu C et al. Bone morphogenetic protein 2 stimulates endochondral ossification by regulating periosteal cell fate during bone repair. Bone.2010; 47(1):65-73.
  • 9van Gestel N,Torrekens S,Roberts SJ,et al.engineering vascularized bone: osteogenic and proangiogenic potential of murine periosteal cells.Stem Cells.2012;30(11 ):2460-2471.
  • 10Amsdorf E J, Jones LM, Carter DR,et aL The periosteum as a cellular source for functional tissue engineering. Tissue Eng Part A 2009;9: 2637-2642.

引证文献1

二级引证文献2

中华骨科杂志
2010年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部