摘要
目的 探讨含纳米银的胶原蛋白-明胶支架材料通过静电吸附层黏连蛋白对周围神经缺损修复的效果.方法 制备纳米银-胶原蛋白支架材料,作为实验组,以不含纳米银的胶原蛋白支架为对照组.将雄性新西兰兔40只随机分为两组,每组20只.将实验组材料和对照组材料分别与层黏连蛋白复合后分组修复兔坐骨神经10 mm缺损.术后30 d通过电生理学、形态学观察和荧光金逆行示踪实验对修复效果进行评估.结果 层黏连蛋白通过静电吸附作用均匀地贴附在实验组支架内表面.兔坐骨神经桥接术后30 d,甲苯胺蓝及透射电镜显示实验组在再生神经纤维数量、神经髓鞘厚度以及被荧光金逆行标记的神经元数量方面均优于对照组.电生理结果：实验组和对照组坐骨神经电位波幅分别为（0.70±0.44）、（0.58±0.37）mV,差异有统计学意义（t=2.803,P=0.012）;神经传导速度分别为（40.7±2.1）、（36.6±4.8）m/s,差异有统计学意义（t=2.427,P=0.031）.结论 纳米银-胶原蛋白-明胶通过静电引力对层黏连蛋白具有较好的吸附作用,而吸附层黏连蛋白的纳米银-胶原蛋白-明胶支架对成年兔坐骨神经10 mm缺损具有较快的修复作用.
Objective To investigate the usefulness of a tissue engineering scaffold made from nano-silver and type-Ⅰ collagen in repair and regeneration of damaged peripheral nerves in adult rabbits.Methods Forty rabbits were randomly divided into an experimental group （nano-sliver-collagen-scaffold with laminin coatings） and a control group （collagen scaffold with laminin）. The 10 mm defects of the rabbit sciatic nerves were repaired respectively in both groups. The functional recovery （regenerated nerve fibers and their myelination status） 30 days after surgery was examined by electrophysiology, Fluoro-Gold retrograde tracing,toluidine blue staining and transmission electron microscopy. Results Compared with the collagen-scaffold without nano-silver in the control group, the nano-silver-containing scaffold showed a higher rate of laminin adsorption, regenerated a nerve with a thicker myelin sheath and improved the nerve conduction velocity and nerve potential amplitude. Fluoro-Gold retrograde labeled the newly grown axons in the spinal cord cortex anterior horn and the dorsal root ganglion. Conclusion The nano-silver-collagen scaffold with laminin coating is effective in promoting axonal regeneration, with a recovery effect comparable to that of autologous nerve graft.
出处
《中华创伤骨科杂志》
CAS
CSCD
2010年第9期855-859,共5页
Chinese Journal of Orthopaedic Trauma
基金
国家自然科学慕金面上项目（30770571）
国家高技术研究发展（863）计划（2002AA216101）
