摘要
目的:建立测定不同斑蝥炮制品及斑蝥虫体各部位中总斑蝥素的含量的方法,探讨炮制对总斑蝥素的影响。方法:HPLC,以甲醇-水(23∶77)为流动相,柱温35℃,流速为1 mL.min-1,检测波长为230 nm。结果:斑蝥总斑蝥素含量远高于药典规定。样品中总斑蝥素含量:生斑蝥饮片>斑蝥药材>米炒斑蝥。头足翅中总斑蝥素的总含量为0.61%。结论:应以总斑蝥素含量控制斑蝥饮片质量。
Objective：The contents in different samples and different parts of the beetle were determined to study the impact of processing blister beetle on the total cantharidin contents.Method：HPLC conditions were as follows：the mobile phase was methanol-water（23∶77）;the column temperature was at 35℃;the flow rate was 1.0 mL.min-1;the detection wavelength was at 230 nm.Result： The total cantharidin contents determined were： unprocessed mylabris〉blister beetle〉mylabris（stir-frying with rice）.The total contents of head,legs,inside and outside wings of blister beetle were 0.61%.Conclusion：For safety,the total cantharidin contents should be used to control the quality of blister beetle.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第15期39-41,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项"中药质量标准和信息化体系建设平台--中药饮片质量标准研究平台"(2009ZX09308-004)
关键词
斑蝥
总斑蝥素
炮制
高效液相色谱法
blister beetle； total cantharidin； before and after processed； HPLC；
