摘要
根据细菌１６ＳｒＲＮＡ基因的高度保守性，设计合成所有细菌、革兰氏阳性细菌及革兰氏阴性细菌的共同引物，采用聚合酶链反应检测已知细菌１３株，三对引物分别扩增的阳性率为１００％，倍比稀释法能检出细菌的最低浓度为４ＣＦＵ·ｍｌ－１，同时检测临床样本４０份，阳性率为６７５％（２７／４０），同期细菌培养阳性率为４５％（１８／４０），二者比较差异有显著性（Ｐ＜０．０５）。结果提示聚合酶链反应检测细菌１６ＳｒＲＮＡ基因具有高度的特异性和敏感性。
According to the high conservative region of 16S rRNA gene in bacteria, PCR primers of the broad range bacteria, gram positive bacteria and gram negative bacteria were synthesized to detect 13 bacterium species and (40) clinical specimens. All the tested bacterium species were positive. The lowest concentration of Escheria Coli detected by serial dilution was 4CFU·ml -1 . The positive rate of PCR(27/40) was higher than that of bacterium culture(18/40). The results indicate that these PCR primers possess high specificity and sensitivity in identifying 16S rRNA gene of bacteria.
出处
《湖南医科大学学报》
CSCD
1999年第2期136-138,共3页
Bulletin of Hunan Medical University
