摘要
在对乌鸡低聚肽的基础理化成分、分子量分布和氨基酸组成进行分析的基础上,从DPPH自由基、羟基自由基和超氧阴离子自由基清除能力以及还原能力4个方面对乌鸡低聚肽的体外抗氧化活性进行了全面的考察。结果显示:乌鸡低聚肽分子量大多为1000u以下(96.74%),含量最高的是140～500u的小肽(78.53%);乌鸡低聚肽具有较强的体外抗氧化能力,抗氧化效果与质量浓度呈正相关,对DPPH自由基、羟基自由基和超氧阴离子自由基的半抑制浓度(IC50值)分别为4.5、4.5和12mg/mL,浓度为9.19mg/mL时的还原能力与0.02mg/mL的抗坏血酸相当。
On the basis of compositions, molecular weight distribution and amino acid composition of black-bone silky fowl （BSF） oligopeptides, the antioxidant activities in vitro of BSF oligopeptides were generally evaluated from four respects by determination of the scavenging activities against DPPH radical, hydroxyl radical and superoxide radical as well as reducing power. Results indicated that the major molecular weight of BSF oligopeptides was less than 1 000 u （96. 74% ）, and small peptides with the molecular weight range from 140 to 500 u were dominated （78.53%）. BSF oligopeptides had strong antioxidant ability in vitro and the antioxidation correlated positively with mass concentration. The median inhibitory concentrations （ICs0 values） of DPPH radical, hydroxyl radical and superoxide radical scavenging activities were 4.5, 4.5 and 12 mg/mL, respectively. Meanwhile, the reducing power of BSF oligopeptides at 9.19mg/mL correlated with that of ascorbic acid at 0.02mg/mL.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2010年第10期19-23,共5页
Food and Fermentation Industries
关键词
乌鸡
低聚肽
氨基酸组成
分子量分布
抗氧化活性
black-bone silky fowl, oligopeptides, molecular weight distribution, amino acid composition, antioxidant activity
