摘要
针对转基因大豆的特异基因CP4-EPSPS(GenBank accession numberAY5966948)设计引物,用LAMP引物在线设计软件设计引物。对反应温度、反应时间、镁离子浓度、Bst DNA聚合酶浓度等扩增条件进行优化,建立了LAMP反应体系,检出限为0.01%。
The specific LAMP primers of Genetically Modified Soybean were designed on the basis of the published sequence of strain CP4-EPSPS（GenBank accession number AY5966948） with the LAMP primer design support software program,using primer explorer software.The reaction conditions wereoptimized including temperature,time,the concentration of Mg2＋,concentration of Bst DNA polymerase etc,the detection limits of LAMP were 0.01%.
出处
《食品科技》
CAS
北大核心
2010年第11期311-315,共5页
Food Science and Technology
关键词
转基因大豆
LAMP
检测
核酸
genetically modified soybean
LAMP
detection
nucleic acid
