摘要
用荧光光谱法在298K研究了Tris-HCl缓冲溶液(pH=7.1)中系列N-烷基-N,N-二(2-羟乙基)-N-甲基溴化铵(烷基链长为C12到C16)与牛血清白蛋白(BSA)的结合作用,考察了表面活性剂结构、BSA浓度对结合作用的影响,分别用Stern-Volmer方程、虚拟结合常数模型探讨了表面活性剂在浓度较低区域与BSA的作用机制.结果表明:三种季铵盐表面活性剂均对BSA内源荧光有猝灭作用,并导致其最大发射波长蓝移;表面活性剂的烷基链越长,Stern-Volmer猝灭常数和虚拟结合常数越大,表面活性剂与BSA的结合作用也越强.
Interactions between a series of N-alkyl-N,N-di（2-hydroxyethyl）-N-methylammonium bromides with alkyl chain from dodecyl to cetyl and bovine serum albumin （BSA） in Tris-HCl buffer solution （pH=7.1） were studied by fluorescence spectroscopy at 298 K. Effects of the surfactant structure and BSA concentration on the interactions were examined. The interaction mechanism between BSA and surfactants at a relatively low concentration range was discussed using the Stern-Volmer equation and a model of the pseudo association constant. Each of the quaternary ammonium surfactants has a fluorescence quenching effect on BSA and leads to a blue shift of the maximum emission wavelength. The Stern-Volmer quenching constant and the pseudo association constant increase, which indicates that the interactions between surfactants and BSA become stronger, as the hydrophobic chain lengths increase.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
2010年第11期2997-3001,共5页
Acta Physico-Chimica Sinica
基金
国家自然科学基金(20673098)
浙江省自然科学基金(Y4080201)~~
关键词
季铵盐表面活性剂
牛血清白蛋白
荧光猝灭
疏水作用
虚拟结合常数
Quaternary ammonium surfactant
Bovine serum albumin
Fluorescence quenching
Hydrophobic interaction
Pseudo association constant
