摘要
目的:检测乙肝病毒表面抗原大蛋白水平的变化,探讨其对乙肝病人诊疗状态的评价价值。方法:1042例乙肝病人以ELISA法进行BHM分组,分别对"大三阳"组、"小三阳"组、HBeAg阴性组、HBV DNA检测阴性组和HBV基因变异组进行HBV-LP测定(ELISA法)及HBV DNA检测(FQ-PCR法)。结果:1042例乙肝病人检测结果显示:L、S、EN及DN组HBV-LP与HBV DNA阳性率分别为73.13%、67.96%,72.73%、42.32%和60.40%、39.88%,除L组(P>0.05)外,其余各组相比差异显著有统计学意义(P<0.01,P<0.05);两种检测方法之间有很好的正相关性(r=0.8420);对HBV DNA检测阴性的196例标本HBV-LP检测的阳性率为68.88%。结论:HBV-LP检测可作为从蛋白水平反映HBV病人体内病毒复制程度的血清学指标,对于HBeAg阴性的乙肝病人HBV-LP检测反映病毒复制优于HBV DNA检测,并与之具有很好互补作用。
Objective：To determinate hepatitis B large surface protein（HBV-LP）for investigating the evaluation value of HBV-LP on the patients with hepatitis B.Methods： Among the groups of L,S,EN and DN HBV-LP（by ELISA） and HBV DNA（by FQ-PCR） were measured respectively in 1042 patients with hepatitis B.Results： There were significant difference between the positive expression rates of HBV-LP and HBV DNA in the groups of S（72.73%,42.32%,P0.01）,EN（60.40%,39.88%,P0.005） and DN（68.88%） and showed a higher positive correlation（r=0.8420） except comparing between the L group（73.13%,67.96%,P0.05）.Conclusion： The HBV-LP detecting would be a serological index that reflect probably the replication of HBV at the protein level,which had an advantage and complementary action between HBV DNA detecting for HBeAg negative patients.
出处
《中国卫生检验杂志》
CAS
2010年第11期2942-2944,共3页
Chinese Journal of Health Laboratory Technology
