摘要
目的研究北京地区Ⅱ／１ｂ型丙型肝炎病毒（ＨＣＶ）包膜蛋白Ｅ２／ＮＳ１高变区１（ＨＶＲ１）序列变异规律及意义。方法应用逆转录巢式ＰＣＲ技术从４６例北京地区Ⅱ／１ｂ型ＨＣＶ感染病人血清中扩增了ＨＣＶ部分包膜区基因片断（ｎｔ１１１９～１２５８），纯化后直接采用双脱氧链末端终止法进行序列分析。结果北京地区Ⅱ型ＨＣＶＨＶＲ１位于氨基酸（ａａ）３８４－４０８位，与有关文献报道（３８３－４１０或４１４）略有差异。ＨＶＲ１序列与ＨＣＶ－Ｊ、台湾株、河北株、ＨＢ－１１相应序列比较，核苷酸同源性依次为４４０％～６６．７％（平均５７．７％），４８．０％～７２．０％（６０．０％），６０．０％～８５．３％（６９．８％）和５６．０％～８１．３％（６８．２％），氨基酸同源性依次为２０．０％－５６．０％（３８．２％），３２．０％～６４．０％（４５．７％），３６．０％～７６．０％（４９．８％）和４０．０％～７６．０％（５５．６％）。本组ＨＶＲＩ内发现６个较保守的氨基酸位点：３８５位Ｔｈｒ，３８９，３９０，４０６位Ｇｌｙ，４０１位Ｓｅｒ，４０３位Ｐｈｅ。结论对ＨＶＲ１序列变异规律及生物学意义的进一步研究有助于ＨＣＶ疫苗的发展。
Objective To study the mpuence deversity of the hypervariable regim 1(HVR1) in theputative envelope protein E2/NS1 of HCV in Beding and its odghbeuring regions. Methods The cDNAs(nt1 119-1258) derived from plasma of 46 patients infected with grnotype Ⅱ/1b Hey were amplified,purified and directly opuenced by using RT-nested polmerase chain reaction(PCR) and dideoxynucleotidechain termination method. Results The HVRI was found in aa384-408 positions of E2 protein, which hadsome differences with other report. Compared with HCV-J, HCV-TW, HCV-HB and HCV-HB11, thenucleotide sequence homologicai rater of these HVR1 were 44.0%-66.7%(57.7%), 48.0%-72.0%(60.0%), 60.0%-85.3%(69.8%), and 56.0%-81.3%(68.2%) respectively, and the amino acid sequence homological rates were 20.0%-56.0%(38.2%), 32.0%-64.0%(45.7%), 36.0%-76.0%(49.8%) and 40.0%-76.0%(55.6%) respectively. There were 6conserved amino acids in HVR1:AA385(Thr), AA389, 390, 406(Gly), AA401(Ser) and AA403(Phe). Twohighly conserved amino acids were only found in this paper. Conclusion The further study of the diversity of HVR1 and its biological significance will be beneficial to the development of HCV vaccine.
出处
《中华肝脏病杂志》
CAS
CSCD
1999年第2期88-90,共3页
Chinese Journal of Hepatology
基金
全军九五课题基金!96M174
关键词
丙型肝炎病毒
高变区1
序列分析
Hepatitis C virus Diversity HVR1 Sequence analysis
