摘要
目的探讨PMA诱导THP-1源分化成巨噬细胞后不同时间(0、12、24、48、72 h)MMP-1的蛋白和mRNA表达。方法采用佛波醇诱导THP-1细胞分化成巨噬细胞。用酶联免疫吸附实验和逆转录-聚合酶链反应测定不同时间MMP-1蛋白和mR-NA的表达。结果在24 h MMP-1蛋白和mRNA的表达增加(0.545 0±0.008 26,0.048 6±0.010 65;0.118 0±0.013 00,0.091 7±0.008 15;0.190 0±0.001 80,0.165 8±0.784 70;0.142 3±0.116 40,0.127 7±0.009 20;0.095 5±0.002 29,0.054 6±0.022 15;P<0.05,P<0.01)。结论 THP-1细胞分化成巨噬细胞后,在24 h MMP-1蛋白和mRNA表达最高,24 h可能降解斑块胶原纤维最强,促进AS的病变的发生和发展。
Aim To investigate the expression of MMP-1 protein and mRNA at different time（0,12,24,48,72） on PMA stimulating THP-1to be macrophages.Methods THP-1 cells differentiated into macrophages induced by PMA.MMP-1 protein and mRNA expres-sion was detected by enzymelinked immunosorbent assay and reverse transcription-polymerase chain reaction at different times.Results The expression increased at 24-hour about MMP-1 protein and mRNA（0.545 0 ± 0.008 26,0.048 6 ± 0.010 65;0.118 0 ± 0.01300,0.091 7 ± 0.008 15;0.190 0 ± 0.001 80,0.165 8 ± 0.784 70;0.142 3 ± 0.116 40,0.127 7 ± 0.009 20;0.095 5 ± 0.002 29,0.054 6 ± 0.022 15;P 0.05,P 0.01）.Conclusion After THP-1 cells differentiate into macrophages,MMP-1 expression were significantly increased at 24 hour,and collagen fibers of plaque may be the strongest degradation by MMP-1 at 24 hours in order to promote the occurrence and development of atherosclerosis lesions.
出处
《安徽医药》
CAS
2010年第12期1410-1412,共3页
Anhui Medical and Pharmaceutical Journal
