摘要
目的观察不同长度时间单肺通气后,两侧肺的氧合指数及氧化应激水平的变化。方法将健康日本大耳白兔24只随机分为4组:对照组(C组,n=6)双肺通气2h;右侧单肺通气组(O1组、O2组及O3组,n=6)单肺通气时间分别为1h、2h、3h,随后恢复双肺通气1h。在实验结束前开胸,取动脉血做血气分析并测定氧合指数;取左右肺组织检测MPO、MDA、SOD。结果各组氧合指数比较,表现为逐步降低,O2组、O3组与O1组相比较明显降低(P<0.01),O1组与C组相比较明显降低(P<0.01)。各组MPO活性的变化表现为C组组内左右侧相比较差异无统计学意义(P>0.05),O1组、O2组和O3组组内左右侧比较则左侧明显增高(P<0.01);各组间右侧比较表现为逐步升高,其中O3组右侧与O1组右侧相比较明显增高(P<0.01),O1组右侧与C组右侧相比较明显增高(P<0.01);各组间左侧相比较亦表现为逐步升高,其中O2组左侧、O3组左侧与O1组左侧相比较明显增高(P<0.01),O1组左侧与C组左侧相比较明显增高(P<0.01)。各组SOD和MDA的变化与之相应。结论长时间单肺通气可以导致不均一性肺损伤,可能与氧化应激水平的变化有关。
Objective To investigate the changes of oxidative stress and oxygenation index after one-lung ventilation for different durations on the rabbit models. Methods 24 white Japanese rabbits were divided into 4 groups （ n = 6 ）. The control group was performed two-lung ventilation for 2 h. The groups of OI ,O2 and O3 were performed one-lung ventilation for 1 h,2 h and 3 h respectively,which were followed with two-lung ventilation for 1 h. Lung separation was achieved with a artificial double-lumen tube. The blood samples were drawn from femoral artery to get the oxygenation indexes. MDA, SOD and MPO were measured with corresponding methods. Results MPO were significant differences in left lung compared to right lung in the groups of O1 , O2 and 03, though no significant differences were noted in the group C. Comparison of the right lungs of the four groups documented significant increase of MPO. Compared with O1 group, MPO in O3 group was significantly elevated（ P 〈 0.05 ）. The changes in the left lung were similar and more severe to the right ones. Compared with C group, MPO in O1 group was significantly eIevated（ P 〈 0.01 ）. Compared with 01 group, MPO in O2 and O3 groups were significantly elevated（ P 〈 0. 01 ）. Similar changes of oxygenation index .MDA and SOD were found in all the groups. Conclusion OLV could cause lung injury by a complex process in the rabbit model. Oxidative stress played an important role in the pathogenesis of OLV-induced lung injury.
出处
《中华全科医学》
2010年第12期1492-1493,共2页
Chinese Journal of General Practice
基金
广东省自然科学基金博士启动项目(8451503102001821)
关键词
单肺通气
肺损伤
氧化应激
One lung ventilation
Lung injury
Oxidative stress
