摘要
目的观察p38激酶抑制剂SB203580和牛磺酸对烧伤后心肌细胞心肌肌钙蛋白T(cTnT)、肿瘤坏死因子α(TNFα)的影响。方法将原代培养的心肌细胞在缺氧、10%烧伤血清条件下培养,建立心肌细胞损伤模型。随机分为A、B、C、D组。A组为对照组,B组加入终浓度为20 mmol/L牛磺酸,C组加入终浓度为10μmol/L的SB203580,D组加入终浓度为20 mmol/L牛磺酸及10μmol/L的SB203580。用酶联免疫法及放射免疫法分别检测各组培养1、3、6、12 h培养液中的cTnT和TNFα。结果从培养3 h开始,B、C、D组培养液cTnT、TNFα水平均比A组低(P均<0.05);D组比B、C组低(P均<0.05),B、C组相比P均>0.05。结论 牛磺酸和SB203580均可降低烧伤后心肌细胞cTnT、TNFα,二者联用具有协同作用。
Objective To observe the effects of taurine（Tau） and SB-203580（p38 kinase inhibitor） on TNF-α and cTnT levels in cardiomycytes injury model with hypoxia and burn serum treament.Methods Neonatal rats cardiomycytes were cultured and divided into five group as follow：control group,hypoxia and burn serum treament group,Tau group,SB-203580 group,Tau＋SB-203580 group.Except the control group,the other four groups cardiaomyocytes that were cultured at different hypoxia stage（1,3,6,12 h）.Enzyme-linked immunosorbent assay and radioimmunoassay were used to detcct the levels of cTnT and TNF-α in culture medium of each group.Result After 3 h hypoxia,the levels of cTnT and TNF-α in culture medium of each experiment group changed significanly.Each experiment group were significanly higher than control group（P〈0.01）.Tau group,SB-203580 group,Tau＋SB-203580 group lower than hypoxia and burn serum treament group（P0.01）.Tau＋SB-203580 group was lower than Tau group（P〈0.05）.Tau group and SB-203580 group had no significant difference.Conclusion Tau and SB-203580 can reduce the TNF-α and cTnT levels in cardiomycytes with hypoxia and burn serum treament.
出处
《山东医药》
CAS
北大核心
2010年第38期11-12,共2页
Shandong Medical Journal
基金
江西省教育厅科研基金资助项目(2007331)
