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一种非精氨酰-甘氨酰-天冬氨酸肽对血小板聚集和ERK1/2磷酸化作用的研究 被引量：3

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摘要 目的观察一种非精氨酰-甘氨酰-天冬氨酸(Arg-Gly-Asp,RGD)肽(P1c,专利公开号:101497656)对血小板聚集的影响和细胞外信号调节蛋白激酶1/2(ERK1/2)磷酸化的作用。方法固相合成法制备P1c;将不同浓度(0～2.6 mmol/L)的P1c加入富血小板血浆中,以二磷酸腺苷(ADP)为诱导剂,检测血小板聚集率;用western blot检测小肽干预后对总ERK1/2和p-ERK1/2的表达。结果 P1c以浓度依赖方式抑制血小板的聚集;其对总ERK1/2的表达无影响,而显著降低血小板p-ERK1/2的表达(P<0.05)。结论 P1c可能通过抑制ERK1/2的磷酸化而减少血小板的聚集。
作者 王喜洪 林江 张粉梅 陈菊 芦慧霞 吴国球
机构地区 东南大学附属中大医院检验科 江阴市第一人民医院检验科
出处 《临床检验杂志》 CAS CSCD 北大核心 2010年第6期444-446,共3页 Chinese Journal of Clinical Laboratory Science
基金 国家自然科学基金(30970809)
关键词 精氨酰-甘氨酰-天冬氨酸 血小板聚集 细胞外信号调节蛋白激酶1/2 磷酸化
分类号 R446.11 [医药卫生—诊断学]
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参考文献2

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共引文献6

同被引文献48

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临床检验杂志
2010年 第6期

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