摘要
为了提高紫杉木霉Trichoderma taxi ZJUF0986突变株UL60-11木霉菌素的产量,采用单因素试验筛选出菌株UL60-11生长及产素的最佳碳氮源为葡萄糖、淀粉、蛋白胨、酵母粉、酒石酸铵;通过均匀设计与正交试验相结合优化出最佳培养基配方:葡萄糖23g、淀粉15g、蛋白胨5g、酵母粉3g、酒石酸铵2g、硫酸镁0.25g、磷酸二氢钾1.0g、氯化钠0.2g、硝酸钙0.4g。在最适工艺条件:初始pH4.0、温度25℃、种龄48h、10%接种量1、00ml/500ml的装液量和150r/min下摇瓶发酵试验,木霉菌素产量达到632.16μg/ml,比优化前提高32%。
To improve the trichodermin yield by the mutant strain UL60-11 of Trichoderma taxi ZJUF0986,fermentation conditions were optimized by single factor and uniform design with orthogonal test.Single factor test indicated glucose and starch were the best carbon sources,and peptone,yeast powder and ammonium tartrate were the best nitrogen sources.The optimal medium was composed of 23 g glucose,15 g starch,5 g peptone,3 g yeast extract,2 g ammonium tartrate,0.25 g magnesium sulfate,1.0 g potassium dihydrogen phosphate,0.2 g sodium chloride and 0.4 g calcium nitrate by uniform design and orthogonal test.Under the optimal culture conditions of temperature 25 ℃,initial pH 4.0,inoculation volume of 10% with 48 h cultured seeds,medium volume 100 ml in 500 ml flask and rotation speed 150 r/min,the yield of trichodermin reached 632.16 μg/mL,increased 32% compared with that under the original fermentation conditions.
出处
《中国生物防治》
CSCD
北大核心
2010年第4期486-491,共6页
Chinese Journal of Biological Control
基金
浙江省重大科技攻关项目(2006C12008)
公益性行业(农业)科研专项项目(200903052)
关键词
紫杉木霉
木霉菌素
发酵
优化
Trichoderma taxi
trichodermin
fermentation
optimization
