摘要
目的用高效液相色谱法测定清肺抑火丸中大黄酸、大黄素、大黄酚、大黄素甲醚的含量。方法 Dia-monsil-C18柱,甲醇-0.2%磷酸(85∶15);流速为1.0 mL.min-1,检测波长254 nm,柱温为室温。结果大黄酸、大黄素、大黄酚、大黄素甲醚的平均回收率分别为100.7%、97.4%、104.0%、103.6%,RSD分别为2.10%(n=6)、1.73%(n=6)、0.12%(n=6)、1.04%(n=6)。结论该法可用于清肺抑火丸的质量控制。
Objective A HPLC method was established for the determination of rhein,emodin,chrysophanol,and physcion in Qingfei Yihuo Pills.Methods A Diamonsil-C18 column was used with mobile phase of methanol-0.2% phosphoric acid solution（85 ∶ 15）at 254 nm.The flow rate was 1.0 mL·min^-1.Results The average recovery of rhein,emodin,chrysophanol and physcion was 100.7 %,97.4 %,104.0 %,103.6 %,respectively.And the RSD were 2.10 %（n=6）,1.73 %（n=6）,0.12 %（n=6）,1.04 %（n=6）,respectively.Conclusion The method can be used for quantitative determination of the preparation.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第6期646-648,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省自然科学基金资助项目(5002841)
