摘要
从养殖水体中筛选出1株对亚硝酸盐具有高效降解能力、增殖速度快、稳定和安全的优良菌株,经细菌学常规检测和16SrRNA序列分析确定为巨大芽孢杆菌(Bacillus megaterium),并命名为SZ-3。对SZ-3菌株降解条件的单因素实验发现,其最佳降解条件是:pH7.0,温度33℃,降解所需的最低葡萄糖含量为0.01mg/L,在24h内对亚硝酸盐的降解率达69.58%。在亚硝酸盐质量浓度为5mg/L的100L污染水体养殖实验中,添加1000mL含量为1×108CFU/mL的SZ-3培养菌液离心后的纯菌体,在28℃的水温条件下经96h,SZ-3菌株对亚硝酸盐的降解率可达91.78%。表明该菌株对污染水体中的亚硝酸盐具有较强的降解效果。本研究旨在为研发高效实用的降解亚硝酸盐微生态活性菌奠定基础。
An efficient,rapid multiplicative,stable and safe nitrite-degradating strain was selected from aquatic water.According to the conventional physiological,biochemical measurement and the 16S rRNA sequence analysis,the strain was identified as Bacillus megaterium and named SZ-3.Based on the single-factor optimization tests to the strain,the optimum degradation conditions are found as follows,the optimal pH value is 7,the perfect temperature is 33 ℃ and the minimum glucose require ment of degradation is 0.01 mg/L.Under these circumstances,the degradation rate of SZ-3 strain to nitrite came up to 69.58% in 24 h.In breeding test of 100 L polluted water in which the concentration of nitrite is 5 mg/L,the degradation rate of SZ-3 strain on the nitrite achieved at 91.78% for 96 h in 28 ℃ via adding the pure thalli of SZ-3 which was obtained by centrifugated 1 000 mL（1×108 CFU/mLl） culture fluid.All of these tests above indicate that the strain can degrade the nitrite in polluted water efficiently.
出处
《中国水产科学》
CAS
CSCD
北大核心
2010年第6期1264-1271,共8页
Journal of Fishery Sciences of China
基金
教育部<长江学者和创新团队发展计划>创新团队基金资助项目(IRTO848)
关键词
菌株筛选
巨大芽孢杆菌
亚硝酸盐
污染水体
screening of microorganisms
Bacillus megaterium
nitrite
pollution water
