摘要
目的观察比较新鲜羊膜和保存羊膜移植在兔角膜急性碱烧伤眼表重建中的作用,并探讨肿瘤坏死因子α(TNF-α)和血小板源性生长因子(PDGF)的疗效。方法建立兔角膜急性碱烧伤模型,设左眼为对照组,右眼为治疗组,并于24h内行新鲜羊膜或保存羊膜移植术(12眼/组)。术后观察双眼形态及角膜新生血管变化,酶联免疫吸附法检测房水中TNF-α的含量,免疫组化法检测角膜组织中PDGF-BB蛋白的表达情况。结果与对照组比较,治疗组炎症反应轻,角膜溃疡范围小,上皮再生速度快,新生血管长度短(P<0.05)。治疗组房水中TNF-α含量低于同期保存羊膜移植组和对照组,且下降速度快,角膜组织中PDGF-BB蛋白表达升高(P<0.05)。与保存羊膜移植组比较,新鲜羊膜移植组炎症反应更轻,TNF-α下降速度更快(P<0.05),PDGF-BB表达水平更高(P<0.05)。结论在角膜急性碱烧伤眼表重建中,新鲜羊膜较保存羊膜效果更好,其作用与抑制TNF-α水平、促进PDGF表达有关。
Objective To compare the therapeutic effect of fresh amniotic membrane transplantation（f-AMT） and preserved amniotic membrane transplantation（p-AMT） in ocular reconstruction of early corneal alkali burns model and to explore the role of tumor necrosis factor α（TNF-α） and platelet derived growth factor（PDGF） in the corneal reparation.Methods Twenty-four rabbits（48 eyes） were used to establish corneal alkali burn models with 0 mmol/L sodium hydroxide.Within 24 hours,f-AMT or p-AMT was performed in the right eyes（12 eyes/group,therapy group）,and the left eyes served as controls.The morphologic changes of the eyes and the neovascularization（CNV） length at the top of corneal surface were observed.TNF-α concentration of aqueous humor from the anterior chamber was measured by enzyme linked immunosorbent assay method after AMT procedure.Also,the PDGF-BB（a marker of PDGF） protein expression of corneal tissue was observed by immunohistochemistry method.Results Compared with those in control group,the AMT eyes showed moderate corneal inflammation,distinctive ulcer boundary,quick epithelium regeneration and shorter CNV length.In therapy group,TNF-α concentration was lower significantly and decreased more quickly than that in control group（P0.05）.The PDGF-BB protein expression increased after ATM procedure.Comapred with therapy group,f-AMT group had milder inflammatory reaction,lower TNF-α concentration and higher PDGF-BB expression than those in p-AMT group（P0.05）.Conclusion Fresh amniotic membrane transplantation is better than preserved amniotic membrane transplatation in ocular reconstruction of early-stage corneal alkali burns,which may be related to its inhibition of TNF-α concentration and increase of PDGF expression.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2010年第6期752-755,769,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科学研究发展计划项目(No.2003K10-G29)~~
关键词
碱烧伤
羊膜移植术
肿瘤坏死因子-α
血小板源性生长因子
alkaline burn
amniotic membrane transplantation
tumor necrosis factor α
platelet derived growth factor
