摘要
为探讨急性坏死性胰腺炎（ＡＮＰ）时肠道菌群变化的规律，经主胰管注入牛磺胆酸钠和胰蛋白酶复制犬ＡＮＰ模型，而后对模型犬肠粘膜及盲肠内容物的菌群进行定性定量分析，对血及脏器作细菌培养。结果：ＡＮＰ犬肠粘膜及盲肠内容物中大肠杆菌计数均明显升高（Ｐ＜０．０５，Ｐ＜０．０１），而双歧杆菌和乳杆菌计数则明显减少（Ｐ＜０．０１）；血浆内毒素水平升高１～２倍；血及脏器细菌培养阳性率为１００％；检出菌种是以大肠杆菌为主的肠道Ｇ－菌。上述实验结果提示：ＡＮＰ时肠道菌群严重失调，生物屏障损伤在ＡＮＰ继发性胰腺感染中可能有重要作用。
To observe the changes of intestinal bacteriology in acute necrotizing pancreatitis (ANP). Dog ANP model was induced by injection of sodium taurocholate with trypin into the pancreatic duct. All dogs were sacrificed on the seventh postoperative day, mucosal and luminal microflora of intestine were analyzed quantitatively. The blood and organs were collected for culture. The results showed that population levels of E.coli in the intestinal mucosa and the content in cecum of the ANP dogs showed much higher level than those of the controls ( P <0.01 or P <0.05), while bifidobacterium and lactobacillus were decreased significantly ( P <0.01), resulting in reversal of bifidobacterium/ E.coli ratio. Blood levels of endotoxin were 1-2 times higher in ANP group as compare with the controls. The positive rate of blood and organs were 100% in ANP dogs. E.coli were the major bacteria cultured. The results indicated that microecological disturbance could take place after the onset of ANP, which may take an important role on pancreatic infection complicating ANP.
出处
《中国普外基础与临床杂志》
CAS
1999年第3期131-133,共3页
Chinese Journal of Bases and Clinics In General Surgery
