摘要
目的探讨RASSF1A基因对胃癌SGC7901细胞蛋白表达谱的影响。方法建立SGC7901和RASSF1A-SGC7901细胞双向凝胶电泳图谱,筛选和鉴定部分分离较好的差异蛋白质点。RT-PCR验证差异基因mRNA的表达。结果建立了分辨率较高、重复性较好的RASSF1A-SGC7901细胞和SGC7901细胞的二维凝胶电泳图谱。筛选出8个差异蛋白质点,包括galectin-1,TRP-14,ACBP,PSMB5,PSMB4,TIM,vimentin及CD79α。RASSF1A过表达诱导了胃癌细胞SGC7901的galectin-1、TRP-14、ABCP的mRNA表达上调。结论 galectin-1,TRP-14及ABCP可能参与了RASSF1A抑制胃癌细胞SGC7901的生长。
Objective To stdy the differential protein expression influensed by RASSF1A gene in human gastric cancer cell line SGC7901.Methods The proteins of SGC7901 and RASSFIA-SGC7901 were isolated by two-dimension gel electrophoresis （2-DE）.Two-DE map was established by Coomassie brilliant blue staining.The well differentially expressed protein spots were screened by Image Master imaging.The specific protein spots were identified by peptide mass fingerprints using matrix-assisted laser desorption/time of flight mass spectrometry （MALDI/TOF/MS）and through searching database using Mascot software.Results Eight differential protein spots were screened and identified.They included galectin-1,TRP-14,ACBP,PSMB5,PSMB4,TIM,vimentin and CD79α.Overexpression of RASSF1A up-regulated the mRNA expression of galectin-1,TRP-14 and ABCP in SGC7901.Conclusions Galectin-1,TRP-14 and ABCP may be involved in growth inhibition induced by RASSF1A in SGC7901.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2010年第10期1080-1084,共5页
China Journal of General Surgery
