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水红花子对2/3肝切除PH小鼠肝再生和骨髓细胞增殖的影响 被引量:2

Effects of fructus Polygoni orentalis on cell proliferation of bone marrow cells in partially hepatectomized mice
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摘要 目的:研究水红花子对部分肝切除小鼠骨髓细胞增殖的影响。方法:参照文献方法制备小鼠2/3肝切除模型。给药7d后处死动物,摘取肝脏,计算肝指数;分离小鼠骨髓细胞,流式细胞仪检测DNA含量和细胞周期分布。结果:水红花子3.6、1.8 g/kg使肝切除小鼠骨髓细胞DNA含量降低,水红花子3.6、1.8、0.9 g/kg使模型鼠G0/G1期细胞数量增加,而G2/M和S期细胞减少,具有剂量依赖性。PH后小鼠的体重增加缓慢,术后7d时模型鼠的体重和残肝重量明显低于正常对照组。水红花子给药组小鼠的体重和残肝重量明显低于正常对照组,但和模型组比较没有显著性差异。结论:水红花子抑制肝切除小鼠骨髓细胞的增殖。 Objective:To explore the effect of fructus Polygoni orentalis(水红花子) on DNA content and cell cycle of bone marrow cells in partially hepatectomized(PH) mice.Methods:Healthy and PH mice which were subject to PH were divided into 5 groups: NS group,control group and fructus Polygoni orentalis groups,respectively,then were treated ig with 0.9% NaCl,and fructus Polygoni orentalis 3.6,1.8,0.9 g/kg daily,respectively.Sacrifice mice and remove the livers for calculating index of liver,bone marrow cells were isolated and detected by flow cytometer(FCM) after 7 days administration.Results:DNA content was decreased by fructus Polygoni orentalis(P〈0.01),the proportion of G0/G1 phase were increased,and G2/M and S phase were decreased(P〈0.01).Body weight(BW) of mice added slowly after PH 7 days,and the weight of residual liver lower than that of NS group.BW of fructus polygoni orentalis 0.9 g/kg obviously decreased,and index of liver of fructus Polygoni orentalis 3.6 g/kg male mice higher than that of control group(p〈0.05).Conclusion: Fructus Polygoni orentalis could inhibit the proliferation of bone marrow cells in PH mice.
出处 《中药药理与临床》 CAS CSCD 北大核心 2010年第5期89-91,共3页 Pharmacology and Clinics of Chinese Materia Medica
基金 河南省自然科学研究计划项目(2008A180013) 河南师范大学科研启动项目(0714) 河南省科技攻关项目(082102310105) 河南省基础与前沿项目(082300453201)
关键词 水红花子 部分肝切除小鼠 骨髓细胞 DNA含量 细胞周期 肝指数 fructus Polygoni orentalis(水红花子); hepatectomized mice; bone marrow cells; DNA content; cell cycle; live index;
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