摘要
目的:4NQO饮水法建立舌鳞癌小鼠模型,观察舌黏膜病变形态学及其肿瘤特异性抗原性的变化特征;探索建立模型理想的4NQO浓度。方法:Balb/c小鼠70只,随机分为A(30只)、B(30只)、C(10只)组,分别用0.010%、0.005%的4NQO水溶液与蒸馏水喂养,A、B组各分为A1(20只)、A2(10只)与B1(20只)、B2(10只)两组。12周开始每2周处死A1与B1小鼠,32周后处死全部小鼠,舌黏膜镜检。观察不同阶段病变舌黏膜的形态学变化及MAGEA1、MAGEA3蛋白表达。结果:A、B组分别于9、12周开始出现舌黏膜的轻度变化,在19、24周出现黏膜的重度改变。实验结束时,A2组小鼠(死亡1只)的浸润癌、原位癌、重度非典型增生分别为7、1和1只,B2组则分别为6、2和2只。其中A2组并发食管鳞癌与食管平滑肌肉瘤各1只。正常舌黏膜、轻-中度、重度非典型增生、舌鳞癌组织的MAGEA1阳性率分别为0、0、30%和55.2%,P=0.000;MAGEA3阳性率分别为10.0%、10.0%、40.0%和48.3%,P=0.055。结论:肿瘤特异性抗原MAGEA1、MAGEA3表达是舌鳞癌发生的伴随事件,与黏膜的形态学改变有相似的过程。0.010%、0.005%的4NQO水溶液均可成功建立舌鳞癌小鼠模型。
OBJECTIVE：To establish the mouse model of tongue squamous cell carcinoma（SCC） induced by drinking water solution of 4-nitroquinoline-1-oxide（4NQO）and observe the characteristic changes of tongue mucosa morphology and the expression of MAGEA1,MAGEA3 and explore the optimal density of 4NQO to establish the model. METHODS： Seventy Balb / c mice were randomly divided into group A（30）,B（30）and C（10）. The mice of group A and B drank respectively 0.005% and 0.010% water solution of 4NQO and distilled water was administered to group C. Group A and B were respectively divided into two subgroups A1（20）,A2 （10） and B1（20）,B2（10）. From the 12th week,two mice were fortnightly selected to dissect every two weeks in group A1 and B1. The rest mice were put to death to dissect until the 32th weeks and observe the changes of morphology and the expression of MAGEA1,MAGEA3. RESULTS： The mice of A,B groups appeared respectively tongue mucosal dysplasia after 9th,12th week and showed severe dysplasia （SDP） after 19th,24th week. When the experiment was over,The mice of group A2 showed early invasive carcinoma（EIC） 7 cases,in situ carcinoma （ISC）1 case,SDP 1 case,besides esophageal squamous cell carcinoma 1 case,esophageal leiomyosarcoma 1 case,and the dead 1 case. The mice of B2 group showed EIC 6 cases,ISC 2 cases,and SDP 2 cases. The positive expression of MAGEA1 in normal tongue mucosa,mild-moderate dysplasia,SDP,SCC were respectively 0,0,30% and 55.2% （P=0.000）,and those of MAGEA3 were respectively 10.0%,10.0%,40.0%,48.3%（P=0.055）. CONCLUSIONS： The expressions of MAGEA1,MAGEA3 accompany with the carcinogenesis in the establishment of the mouse model of tongue SCC,and which are similar to the characteristic changes of morphology,and 0.010%,0.005% solution of 4NQO can successfully induce the mouse model of tongue SCC.
出处
《中华肿瘤防治杂志》
CAS
2010年第19期1510-1513,共4页
Chinese Journal of Cancer Prevention and Treatment
