摘要
目的探讨胃粘膜癌变过程中幽门螺杆菌（Ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）感染与ｐ５３，ｃｅｒｂＢ２基因表达的关系．方法浅表性胃炎１６例，肠上皮化生２２例，异型增生１４例，早期胃癌１８例及进展期胃癌４０例作为研究对象．用ＷａｒｔｈｉｎＳｔａｒｙ银染色法检测Ｈｐ，用免疫组化Ｓｐ法检测ｐ５３和ｃｅｒｂＢ２的基因表达产物．结果Ｈｐ，ｐ５３，ｃｅｒｂＢ２在浅表性胃炎的检出率各为５００％，００％，００％；在肠上皮化生的检出率各为５９１％，２２７％，１３６％；在异型增生的检出率各为８５７％，６４３％，２８６％；在早期胃癌的检出率各为１６７％，３３３％，１１１％；在进展期胃癌的检出率各为５０％，５２５％，５５０％；在癌旁粘膜的Ｈｐ检出率为８６７％；在癌前病变中，Ｈｐ阳性组的ｐ５３，ｃｅｒｂＢ２表达率均高于Ｈｐ阴性组．结论Ｈｐ感染参与了胃癌前病变的发生与发展；Ｈｐ感染可引起野生型ｐ５３基因失活和ｃｅｒｂＢ２基因激活，从而导致胃粘膜的癌变．
AIM
To study the relationship between Helicobacter pylori infection and p53, c erbB 2 gene
expression in carcinogenesis of gastric mucosa. METHODS Sixteen cases of superficial
gastritis, 22 intestinal metaplasia, 14 dysplasia, 18 early gastric cancer and 40 advanced
gastric cancer were studied. Hp was detected by Warthin Starry Ag staining, and p53, c
erbB 2 gene expression were detected by Sp immunohistochemical method. RESULTS Hp,
p53 and c erbB 2 positive rates were 50 0%, 0 0%, and 0 0% in superficial gastritis, 59
1%, 22 7% and 13 6% in intestinal metaplasia and 85 7%, 64 3% and 28 6% in dysplasia
respectively. The positive rates were 16 7%, 33 3% and 11 1% in early gastric cancer and 5
0%, 52 5% and 55 0% in advanced gastric cancer. The positive rate of Hp in adjacent cancer
mucosa was 86 7%. In precancerous lesions, the positive rates of p53 and c erbB 2 in
Hp (+) group were higher than in Hp (-) group. CONCLUSION Hp infection plays a certain
role in the pathogenesis of gastric precancerous lesions. Hp infection causes the inactivation
of wild type p53 gene and activation of c erbB 2 gene, resulting in the occurrence of
gastric cancer.
出处
《世界华人消化杂志》
CAS
1999年第4期313-315,共3页
World Chinese Journal of Digestology
关键词
胃肿瘤
幽门螺杆
P53基因
C-ERBB-2基因
stomach neoplasms
Helicobacter pylori
p53 gene
c
erbB 2 gene
gene expression
immunohistochemistry
