摘要
目的:探讨125I粒子对低分化大肠腺癌的抑制作用及其可能的机制.方法:以人低分化结肠癌HCT-116细胞的荷瘤小鼠作为动物模型,随机将其分成对照组和实验组(每组24只),对照组植入空白粒子,实验组植入剂量为14.8MBq的125I粒子,分别在植入后第7天、14天、21天、28天处死裸鼠.计算生长曲线,用免疫组织化学法检测增殖细胞核抗原;以原位末端标记法检测125I粒子诱导肿瘤细胞凋亡的程度;通过透射电镜对比观察肿瘤组织的形态学变化.结果:植入125I粒子第7、14、21、28天后,实验组荷瘤小鼠肿瘤体积均明显小于对照组(第28天:497.3mm3±7.8mm3vs947.2mm3±40.4mm3,P<0.01).同时,实验组荷瘤小鼠肿瘤质量也均明显小于对照组(第28天:1.131g±0.079gvs2.139g±0.094g,P<0.01).实验组的抑瘤率高于对照组(第28天:47.12%vs25.77%).从第21天开始PCNA的表达逐渐降低,在第28天时明显低于对照组,其表达呈弱阳性(+).植入125I粒子14d后,开始出现凋亡小体,且数量逐渐增多,以第28天时达到最大.结论:125I粒子对低分化大肠腺癌的治疗有效,他能抑制肿瘤细胞的增殖生长,诱导肿瘤凋亡.
AIM：To investigate the effect of interstitial implantation of iodine-125 on the growth of adenocarcinoma of the large intestine and to explore potential mechanisms involved.METHODS：Nude mice bearing HCT-116-xenografted tumors were randomly divided into two groups（n = 24 each）：control group andexperimental group.The control group underwent implantation of empty seeds,while the experiment group underwent implantation of iodine-125 seeds（14.8 MBq）.On days 7,14,21 and 28 after implantation,mice were killed to calculate tumor growth,detect the expression of proliferating cell nuclear antigen（PCNA） in tumor tissue by immunohistochemistry,determine the apoptosis of tumor cells by terminal deoxynucleotidyl transferase dUTP nick-end labeling（TUNEL） assay,and observe the ultrastructural changes of tumor cells by transmission electron microscopy（TEM）.RESULTS：On days 7,14,21 and 28 after implantation,both tumor volume and weight were signifi cantly lower in the experiment group than in the control group（tumor volume on day 28：497.3 mm3 ± 7.8 mm3 vs 947.2 mm3 ± 40.4 mm3,P 0.01;tumor weight on day 28：1.131 g ± 0.079 g vs 2.139 g ± 0.094 g,P 0.01）.The reduced rate of tumor growth in the experimental group was higher than that in the control group（on day 28：47.12% vs 25.77%）.The expression of PCNA in the experiment group decreased with time and was significantly lower on day 28 than that in the control group.On day 14,apoptotic bodies began to appear and then gradually increased in number,reaching the peak on day 28.CONCLUSION：Interstitial implantation of iodine-125 seeds induces apoptosis but inhibits cell proliferation in HCT-116-xenografted tumors in nude mice.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第27期2858-2862,共5页
World Chinese Journal of Digestology
基金
云南省应用基础研究联合专项基金资助项目
No.2007C0039R
云南省应用基础研究重点基金资助项目
No.2008CC002~~
关键词
125I粒子
大肠癌
凋亡
增殖细胞核抗原
Iodine-125 seed
Colorectal cancer
Apoptosis
Proliferating cell nuclear antigen
