摘要
通过分析4个完成测序和注释的植物基因组,系统地分离鉴定了97个水稻、玉米、高粱和拟南芥的CCT结构域基因,并对相应蛋白质的结构和基因之间的系统演化关系进行了分析。结果表明:CCT结构域基因的蛋白质结构和特性在不同物种之间具有广泛的变异;不同基因组中CCT结构域基因通过染色体复制扩展了基因家族成员。根据其CCT结构域分为4组,其中1个亚组集中了绝大部分的禾本科CCT结构域基因,该亚组基因可能特异参与了禾本科作物开花时间的调控。基因家族成员的扩展,蛋白质结构的改变以及基因表达模式的变异共同导致了CCT结构域基因家族成员在物种间和物种内的功能分化。
The CCT domain defines a conserved 43～45 amino acids of the 3＇ region of CONSTANS,CO-like,and TOC1.The plant specific CCT domain gene plays a role in light signaling and regulation of photoperiodic flowering.Meanwhile,the evolutionary relationship among family members has not been well studied.In this study,97 CCT domain containing genes were retrieved from four completely sequenced and annotated genomes.Analyses of corresponding protein structure and genetic relationships showed that CCT domain containing protein genes kept a wide range of variation within and among different species;Sizes of CCT domain gene family were increased through chromosome duplication.CCT domain gene family was classified into four subgroups according to the CCT domain.One sub-group gathered most of the grass CCT domain gene,which might be involved in the regulation of flowering time.The functional differentiation within and among species was comprehensively caused by the expansion of gene family members,structural variation of protein and patterns of gene expression.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第6期669-676,共8页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30830064)资助
关键词
CCT结构域基因
染色体复制
蛋白质结构
功能分化
CCT domain gene family
chromosome duplication
protein structure
function differentiation
