摘要
目的了解该院铜绿假单胞菌对β-内酰胺类制剂的耐药情况以及相关耐药基因的携带现况。方法自临床分离的119株铜绿假单胞菌均采用PCR检测耐药基因(TEM、SHV、CTX-M、DHA-1、OXA、CARB、VEB-1、PER-1、GES-2、AmpC、VIM-1、VIM-2、IMP-1、SPM、OprD和IntⅠ)并对检出的耐药基因进行DNA测序。结果 119株铜绿假单胞菌中TEM阳性103株(86.6%),AmpC阳性77株(64.7%),IntⅠ阳性54株(45.4%),OprD缺失54株(45.4%),VIM-2阳性19株(16.0%),CARB阳性14株(11.8%),SHV阳性2株(1.7%),OXA阳性2株(1.7%),DHA-1阳性1株(0.8%),其余基因均为阴性。结论该院铜绿假单胞菌TEM、AmpC、IntⅠ、VIM-2等耐药基因的携带率及OprD缺失均较高,多重耐药严重。
Objective To know situations of Pseudomonas aeruginosa resistant to β-lactams and related coded genes in our hospital. Methods Drug-reslstant genes of 119 Pseudomonas aeruginosa clinical isolates were detected by PCR and DNA sequence, including TEM, SHV, CTX-M, DHA-1, OXA, CARB, VEB-1, PER-1, GES-2, AmpC, VIM-1, VIM-2, IMP-1, SPM, OprD and IntI. Results In 119 Pseudomonas aeruginosa clinical isolates, positive PCR products are TEM, AmpC, integron I, VIM-2, CARB, SHV,OXA and DHA 1 ,respectively 103 strains（86.6%）,77 strains（64.7%）,54 strains（45.4%）, 19 strains（16.0 %）, 14 strains （11.8%） ,2 strains（1.7%） ,2 strains（1.7%） ,1strain（0.8%） and 54 strains are lack of genes coded OprD,the rate is 45.4%. The other genes are negative. Conclusion Detection rates of gene .coded TEM,AmpC,IntI and VIM-2 are high. The situations of multi --drug resistance and the lacking of OprD are serious.
出处
《国际检验医学杂志》
CAS
2010年第11期1221-1223,共3页
International Journal of Laboratory Medicine
关键词
假单胞菌
铜绿
Β内酰胺酶类
抗药性
细菌
Pseudomonas aeruginosa
beta lactamases
drug resistance,bacterial
