摘要
目的建立冬葵果药材中咖啡酸的含量测定方法。方法RP-HPLC法：采用翡纳米科技kromasil ODS C18色谱柱（250 mm×4.6 mm,5μm）,甲醇-1%冰醋酸水溶液（18∶82）等度洗脱;流速：1.0 mL/min,检测波长：330 nm;柱温：30℃。结果咖啡酸在0.027 52~0.344 0μg范围内线性关系良好。平均回收率为98.85%,RSD为2.29%（n=6）,符合定量分析要求。结论结果表明,所建立的方法简便、准确,适用于冬葵果中咖啡酸的含量测定。
Objective To establish a method for the quantitative determination of caffeic acid in Dongkuiguo（Fructus Malvae,Mongolian medicated herb） by RP-HPLC.Methods The procedure of RP-HPLC was performed on the chromatographic column of kromasil ODS C18（250 mm×4.6 mm,5 μm）,and the mobile phase in the isocratic elution was methanol-1% acetic acid（18 to 82）.The flow rate was 1.0 mL/min,the detection wavelength was 330 nm,and the temperature was at 30 ℃.Results Coffeic acid showed a good linear relationship at a range from 0.0275 2 μg to 0.344 0 μg,and the average recovery was 98.85%（RSD=2.29%,n=6）.Conclusion The result of the quantitative determination shows that the method is simple and accurate,which is suitable for the quantitative determination of caffeic acid in Dongkuiguo.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2010年第2期117-119,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
2010版<中国药典>(一部)中药标准科研资助项目(No.YD-028)
关键词
冬葵果
咖啡酸
高效液相色谱法
含量测定
Dongkuiguo（Fructus Malvae
Mongolian medicated herb）
caffeic acid
HPLC
quantitative determination
