摘要
用分值计算法对四批ＳＳ琼脂质量检测，分值均小于质控标准分值５６．１２５。主要问题是抑制大肠杆菌（Ｅｃｏｌｉ）生长和促鼠伤寒沙门氏菌（Ｓｔｙｐｈｉｍｕｒｉｕｍ），痢疾志贺氏菌（Ｓ．ｄｙｓｅｎｉｃｒｉａｅ）生长的能力不够。调整ＳＳ琼脂配方中各试剂的用量进行筛选，结果表明：０．５％胆盐抑制大肠杆菌（Ｅｃｏｌｉ）能力达到分值质控要求，但对鼠伤寒沙门氏菌（Ｓｔｙｐｈｉｍｕｒｉｕｍ）和痢疾志贺氏菌（Ｓ．ｄｙｓｅｎｉｃｒｉａｅ）的生长同样有抑制作用。０．３％胆盐抑制大肠杆菌（Ｅｃｏｌｉ）的能力不明显。０．０７％柠檬酸铁和０，１５％柠檬酸钠有缓和胆盐对鼠伤寒沙门氏菌（Ｓｔｙｐｈｉｍｕｒｉｕｍ）的毒性，但对痢疾志贺氏菌（Ｓｄｙｓｅｎｉｃｒｉａｅ）作用不明显。
Four batches of SS agar were qualified as substandard products with Scoring Method (score < 56.125).The main problem was weak ability in inhibiting the growth of E. coli and promoting the growth of S.typhimurium and S. dysenteriae. Tests for optimizing the formula of SS agar were conducted. Amoung these tests,the contents of some components were variant. The results showed that 0.5% cholate inhibited the growth of E.coli at an ideal level but also inhibited the growth of S. typhimurium and S. dysenterias. 0.3% cholate had noobvious effects in iuhibiting E. coli. 0.070% feric citrate and 0.15% sodium citrate could lessen the toxicity ofcholate to S. typhimurium with no effects in protecting S. dysenteriae.
出处
《微生物学通报》
CAS
CSCD
北大核心
1999年第2期113-116,共4页
Microbiology China
