摘要
将雌性ＳＤ大鼠分为４组，Ａ组交配后第一天起，Ｂ组交配后第１０天起每天在隔离器内被动吸烟２ｈ至第２０天，Ｃ组为普通环境不吸烟对照组，Ｄ组为每天在隔离器内饲养２ｈ不吸烟对照组。实验结果显示，Ａ，Ｂ，Ｃ组胎内活仔体重分别为２．０±０．７，２．８±０．５，３．７±０．４ｇ；宫内发育迟缓（ＩＵＧＲ）发生率分别为８７％，５９．６％，５％；体长分别为２．８４±０．４２，３．２４±０．３０，３．９４±０．２２ｃｍ；尾长分别为０．９６±０．１６，１．０７±０．１４，１．３４±０．１０ｃｍ；胎盘重量分别为０．４８±０．１２，０．５０±０．１１，０．６２±０．４０ｇ；Ｄ组各项指标与Ｃ组比较无显著差异。正常对照Ｃ组和不吸烟对照组Ｄ组胚胎早期吸收或死胎率仅为３．４５％和４．１９％，而被动吸烟的Ａ，Ｂ组分别为３２．４％与１６．１％，均明显高于对照组，表明被动吸烟对胚胎的存活也有很大的威胁。本研究结果提示，利用隔离器和大鼠建立被动吸烟致ＩＵ－ＧＲ动物模型，具有吸烟环境易控制，重复性好，实验周期短。
The pregnant SD rats were divided into 4 groups as follows: group A and group B, passive smoking for 2h everyday in isolator from 1st and 10th day to 20th day after mating; group C, the control group without passive smoking under normal condition; and group D, the control group without passive smoking, placed in isolator for 2h everyday. The results showed that there were significant difference between group A,B, and group C in the average body weight of live fetal rats (2.0±0.71, 2.82±0.50g vs 3.74±0.40g), the incidence of IUGR (87%, 59.6% vs 5%), the average body length (2.84±0.42, 3.24±0.30cm vs 3.94±0.22cm) and the average weight of placenta (0.48±0.12, 0.50±0.11g vs 0.6±0.40g) . There is no difference between control group C and D. The early resorption rate of embryo and fetal death rate of group A and B were 32.4% and 19.2%, which were significantly higher than that of control group C and D (3.45% and 4.19%), this suggests that passive smoking even threaten the survival of fetal rat. These results showed that the IUGR rat model established in isolator was easy to control, could be easily repeated, short in experimental period and high in incidence of IUGR.
出处
《上海实验动物科学》
1999年第1期14-17,共4页
Shanghai Laboratory Animal Science
