摘要
目的 观察桂枝茯苓胶囊对动脉粥样硬化（AS）模型大鼠的影响.方法 SD大鼠随机分为空白组12只和造模组38只.空白组予普通饲料 造模组予高脂饲料＋维生素D3腹腔注射,造模成功后将该组按照随机数字表法随机分为模型组、西药组、中药组各12只,分别予生理盐水、辛伐他汀合卡托普利、桂枝茯苓胶囊灌胃,16周末处死动物.测定血清内皮素-1（ET-1）、一氧化氮（NO）、高敏C反应蛋白（hs-CRP）含量及主动脉细胞间黏附分子-1（ICAM-1）的表达.结果 空白组ET-1、NO、hs-CRP、ICAM-1积分光密度分别为（142.61±25.67）ng/ml、（66.05±8.63）μmol/L、（2.86±0.40）mg/L、（0.29±0.05） 模型组为（182.38±22.96）ng/ml、（28.70±4.49）umol/L、（5.60±0.49）mg/L、（0.58±0.06）,与空白组比较,差异有统计学意义（P=2.47E-4、6.02E-19、1.1lE-12、2.51E-18）.西药组为（154.37±21.11）ng/ml、（45.88±11.36）μmol/L、（3.66±0.34）mg/L、（0.37±0.04）,与模型组比较,差异有统计学意义（P=0.005,1.59E-14,1.87E-5,3.87E-14）.中药组为（152.13±23.23）ng/ml、（57.67±8.96）μmol/L、（3.70±0.40）mg/L、（0.36±0.04）,与西药组比较,NO较高（P=0.002）,ET-1、hs-CRP、ICAM-1积分光密度差异无统计学意义（P=0.81、0.81、0.48）.结论 桂枝茯苓胶囊可改善AS的相关指标.
Objective To observe the anti-atherosclerosis effects of Guizhi Fuling Capsule. Methods 50 healthy SD male rats were randomly divided into 2 groups： normal group （n=12） and AS model group（n=38）. The model of experimental AS rats was established by feeding high cholesterol diet and intraperitoneal injecting VitD3. The normal control group was fed by ordinary diet. AS model group was further divided into 3 groups randomly： AS group（n=12）, control group （n= 12）, and therapy group（n= 12）. The therapy group was given Guizhi Fuling Capsule by intragastric administration.The control group was given simvastatin and captopril by intragastric administration. 0.9% NaCl was given to the AS group instead of Guizhi Fuling Capsule. The rats were killed at the 16th weekend, and their aorta arch and breast aorta were observed.Blood fat and serum levels of Endothelin-1 （ET-1）, Nitric oxide （NO）, high-sensitivity C-reactive protein （hs-CRP） were detected respectively. Immunohistochemistry technology was used for observing the expression of intercellular adhesion molecule- 1 （ICAM- 1 ） in breast aorta. Results The values of ET- 1, NO, hs-CRP and the amount of ICAM- 1 were （142.61 ±25.67）ng/ml, （66.05 ± 8.63） μmol/L, （2.86±0.40）mg/L, （0.29±0.05） in the normal group （182.38±22.96）ng/ml, （28.70±4.49）μmol/L, （5.60±0.49）mg/L, （0.58±0.06）in the AS group （154.37±21.11）ng/ml, （45.88±11.36）μmol/L, （3.66±0.34）mg/L, （0.37±0.04）in the control group and （152.13±23.23）ng/ml, （57.67±8.96）μmol/L, （3.70±0.40）mg/L, （0.36±0.04） in the therapy group respectively. The levels of ET-1, NO, hs-CRP and the amount of ICAM-1 have significant difference between the AS group and the normal group（P=2.47E-4, 6.02E-19, 1.11E-12, 2.51E-18）. There was also significant difference between the control group and the AS group（P=0.005, 1.59E-14, 1.87E-5, 3.87E-14）. The level of NO in the therapy group was higher than that in the control group （P=0.002）. The levels of ET-1, hs-CRP and the amount of ICAM-1 did not have significant difference between the therapy group and the control group（P=0.81, 0.81, 0.48） Conclusion Guizhi Fuling Capsule has effects of Anti-atherosclerosis on rats with AS.
出处
《国际中医中药杂志》
2010年第6期493-495,共3页
International Journal of Traditional Chinese Medicine
基金
河南省科技厅课题（项目编号：4244101149）
