摘要
目的探讨黄芪对宫颈癌患者外周血辅助T细胞1(naive helper T cells,Th1)和辅助T细胞2(Th2)型细胞因子表达失衡的调节作用。方法 30例宫颈癌患者应用黄芪注射液20 mL/d(40 g生药),静脉滴注1周前后采集外周血液标本,同时采集10名健康人的标本做对照。密度梯度离心获取外周血单个核细胞,流式细胞术分析CD4+interferon-γ(IFN-γ)细胞和CD4+interleukin-4(IL-4+)细胞占CD4+T细胞的比例;酶联免疫吸附方法(ELISA)检测细胞培养上清IFN-γ、IL-4的水平;逆转录聚合酶链术(RT-PCR)检测T淋巴细胞转录因子T-bet[(transcription factors T-box expressed in T-cells)]及转录因子[GATA-3 GATA-binding protein-3)]的表达。结果宫颈癌患者CD4+IFN-γ+细胞比例、T-bet mRNA水平及培养上清IFN-γ浓度明显低于健康对照组,CD4+IL-4+细胞比例、GATA3 mRNA水平及培养上清IL-4浓度两组间差异无统计学意义。黄芪治疗后CD4+IFN-γ+细胞比例、T-bet mRNA表达及培养上清IFN-γ浓度明显增高(P<0.05),CD4+IL-4+细胞相关指标未见明显改变。结论宫颈癌患者存在Th1/Th2细胞功能失衡,呈Th2优势应答模式,黄芪治疗可使Th2/Th1平衡向Th1偏移从而发挥抗肿瘤作用。
Objective To investigate the effect of Astragalus in regulating the imbalance between naive helper mononuclear cells by density gradient centrifugation.The positive percentages of CD4＋ interferon-γ（IFN-γ） cell and CD4＋ interleukin-4（IL-4） cell in total CD4＋ cells were measured by flow cytometry;the contents of IFN-γ,IL-4 in T cells（Th1/Th2） cytokines expression in patients with cervical cancer.Methods Thirty patients with cervical cancer received intravenous dripping with 20 mL of Astragalus Injection（AI,contained extract from 40 g of crude drug） per day for 1 week,peripheral blood sample was collected from patients separately before and after treatment for extract culture supernate were detected with ELISA;and the expressions of T-cell transcription factor T-cells（T-bet） and GATA-CD4＋ IL-4 positive cell associated parameters.Conclusion Th1/Th2 cell function imbalance existed in patients with cervical cancer,showing a Th2 predominant reaction mode;AI can regulate the imbalance,offset to Th1,thus to display its anti-tumor effect.binding protein-3（GATA-3） were determined by RT-PCR.The data were controlled by those get from 10 healthy persons.Results CD4＋ IFN-γ positive cell percentage,T-bet mRNA expression level and concentration of IFN-γin supernate were significantly lower in patients than those in healthy control respectively,while CD4＋ IL-4 positive percentage,level of GATA3 mRNA expression and IL-4 concentration in supernate were insignificantly different between the two groups.After AI treatment,the lowered parameters were up-regulated（P0.05）,and no obvious change was observed in the CD4＋ IL-4 positive cell associated parameters.Conclusion Th1/Th2 cell function imbalance existed in patients with cervical cancer,showing a Th2 predominant reaction mode;AI can regulate the imbalance,offset to Th1,thus to display its anti-tumor effect.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2010年第11期1157-1159,共3页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
黄芪
宫颈癌
转录因子
细胞因子
astragalus
cervical cancer
transcription factor
cytokines
