摘要
将烟草中经修饰的Ⅰ类几了质酶和β－1，3-葡聚糖酶cDNA分别加上CaMV35S启动子和NOS终止子，依次插入到同一植物表达载体pBin19上，获得植物双价表达载体pCG－Ⅱ。然后载体pCG－Ⅱ以土壤农杆菌LBA4404介导转化番茄，再生植株的点杂交及PCR扩增证明两种cDNA已随T－DNA同时导入番茄植株中。
The modified tobacco Ⅰ-Chitinase (Chi) and b-1, 3 -Glucanase (Glu) cDNAwith CaMV 35 S promotor and NOS terminator were cloned into pBin19, and a plantbivalent expression vector pCG-Ⅱ was obtained. The pCG-Ⅱ was then transformed intotomato through mediation of Agrobacterium tunefaciens LBA4404. The dot-blot hybridization and PCR amplification analysis demonstrated that the Chi and Glu cDNA were integrated into tomato genomes.
出处
《热带作物学报》
CSCD
1999年第1期29-33,共5页
Chinese Journal of Tropical Crops
